Journal
PLANT DIRECT
Volume 7, Issue 11, Pages -Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/pld3.539
Keywords
annexin; cold stress tolerance; CRISPR/Cas9; rice
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This study provides a comprehensive account of the cloning and functional characterization of the rice annexin gene OsAnn5. The results reveal that OsAnn5 is involved in cold stress tolerance at the seedling stage by participating in the endoplasmic reticulum apparatus. These findings have important implications for understanding plant stress responses and related cellular processes.
Annexins exist widely in plants as multigene families and play critical roles in stress responses and a range of cellular processes. This study provides a comprehensive account of the cloning and functional characterization of the rice annexin gene OsAnn5. The findings reveal that a cold stress treatment at the seedling stage of rice induced OsAnn5 expression. GUS staining assay indicated that the expression of OsAnn5 was non tissue-specific and was detected in almost all rice tissues. Subcellular localization indicated that OsAnn5-GFP (green fluorescent protein) signals were found in the endoplasmic reticulum apparatus. Compared with wild type rice, knocking out OsAnn5 using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins) mediated genome editing resulted in sensitivity to cold treatments. These results indicate that OsAnn5 is involved in cold stress tolerance at the seedling stage.
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