Engineering of two thiamine diphosphate-dependent enzymes for the regioselective condensation of C1-formaldehyde into C4-erythrulose

Article Chemistry, Multidisciplinary

Multilayer Engineering of an Escherichia coli-Based Biotransformation System to Exclusively Produce Glycolic Acid from Formaldehyde

Hye-Jin Jo et al.

ACS Sustainable Chemistry & Engineering (2023)

Article Multidisciplinary Sciences

Engineering a new-to-nature cascade for phosphate-dependent formate to formaldehyde conversion in vitro and in vivo

Maren Nattermann et al.

Summary: Researchers developed a two-enzyme route to activate formate into formyl phosphate and reduce it to formaldehyde, providing a potential tool for synthetic formate assimilation.

NATURE COMMUNICATIONS (2023)

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Review Chemistry, Multidisciplinary

Enzymatic Conversion of CO2: From Natural to Artificial Utilization

Sarah Bierbaumer et al.

Summary: Enzymatic carbon dioxide fixation is important for converting inorganic carbon from the atmosphere into organic biomass. However, the thermodynamics and utilization of CO2 make it challenging for biotechnological applications. This review provides an overview of natural CO2-fixing enzymes, discusses their applications, and suggests strategies for improving efficiency. It also explores the potential of using reduced CO2 derivates and offers a techno-economic perspective on reducing CO2 emissions.

CHEMICAL REVIEWS (2023)

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Review Engineering, Environmental

Valorization of C1 gases to value-added chemicals using acetogenic biocatalysts

Jiyun Bae et al.

Summary: This article discusses the use of acetogenic bacteria as biocatalysts for converting C1 gases into value-added chemicals, providing a comprehensive overview of the process. Various strategies for enhancing product yield and alcohol selectivity during gas fermentation processes are also discussed.

CHEMICAL ENGINEERING JOURNAL (2022)

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Article Chemistry, Multidisciplinary

Glyoxylate carboligase-based whole-cell biotransformation of formaldehyde into ethylene glycol via glycotaldehyde

Hye-Jin Jo et al.

Summary: A novel biocatalytic system was established for the synthesis of industrially relevant C2 chemicals, such as ethylene glycol, from formaldehyde. By improving the substrate access tunnel, the affinity and catalytic efficiency of the thermostable glyoxylate carboligase for formaldehyde were enhanced, leading to the production of ethylene glycol through the condensation of two molecules of formaldehyde into one molecule of glycolaldehyde. The study demonstrated a green approach for the biocatalytic synthesis of ethylene glycol from Cl compounds.

GREEN CHEMISTRY (2022)

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Article Biotechnology & Applied Microbiology

Development of a kinetic model and figures of merit for formaldehyde carboligations catalyzed by formolase enzymes

Nadim Massad et al.

Summary: There is a growing interest in upgrading inexpensive and abundant C-1 feedstocks to higher carbon products. Linear carbon ligation routes are favored due to their simplicity and potential for high carbon efficiencies. This study provides a theoretical framework for comparing the performance of FLS enzymes, which is crucial for designing and implementing novel carbon ligation pathways.

BIOTECHNOLOGY AND BIOENGINEERING (2022)

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Article Biochemistry & Molecular Biology

Revealing Donor Substrate-Dependent Mechanistic Control on DXPS, an Enzyme in Bacterial Central Metabolism

Melanie L. Johnston et al.

Summary: DXPS is essential in bacteria but absent in human metabolism, making it a potential antibacterial drug target; The enzyme possesses unique features that enable selective inhibition strategies and raise questions about its function in bacterial pathogens.

BIOCHEMISTRY (2021)

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Article Chemistry, Multidisciplinary

Enantioselective Reductive Oligomerization of Carbon Dioxide into L-Erythrulose via a Chemoenzymatic Catalysis

Sarah Desmons et al.

Summary: This study reports a novel hybrid chemoenzymatic catalytic process for the transformation of CO2 into C-3 and C-4 carbohydrates with perfect enantioselectivity. By first converting CO2 into formaldehyde and then employing enzymatic transformations, the desired chiral compounds are obtained. This method is of significant importance for the chemical and pharmaceutical industries.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

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Article Chemistry, Multidisciplinary

Design of a synthetic enzyme cascade for the in vitro fixation of a C1 carbon source to a functional C4 sugar

Samed Guener et al.

Summary: This paper focuses on utilizing formaldehyde as a C-1 carbon source for enzymatic C-C bond formation, successfully designing a synthetic enzyme cascade for producing the functional C-4 sugar erythrulose. Through high-throughput combinatorial screening, optimized enzyme variants were developed, and it was shown for the first time that erythrulose can be produced from a C-1 carbon source. Tailored formolase variants were able to convert glycolaldehyde to erythrulose with a 98% theoretical yield, representing the highest achieved in vitro concentration of erythrulose to date.

GREEN CHEMISTRY (2021)

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Article Biochemical Research Methods