Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Volume 45, Issue 2, Pages 372-379Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/21691401.2016.1160403
Keywords
Antibacterial; green synthesis; Rheum palmatum; SEM; silver nanoparticle; XRD
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Funding
- National Institute of Animal Science, RDA [PJ010168]
- Rural Development Administration (RDA), Republic of Korea [PJ010168032016] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In the present study, silver nanoparticles (AgNPs) were synthesized by using aqueous root extracts of Rheum palmatum and characterized by various spectroscopic methods. The nanoparticles were found to be in hexagonal and spherical shapes. The average particle size was found to be 121 +/- 2 nm with zeta potential values of -21.6mv by dynamic light scattering (DLS) method. Gas chromatography-mass spectrometry (GC-MS) analysis of R. palmatum revealed 35 compounds. The synthesized AgNPs showed significant activity against Staphylococcus aureus and Pseudomonas aeruginosa with IC90 values of 15 mu g/ml and IC50 values of 7.5 mu g/ml, respectively. The protein leakage level was high and morphological changes occurred in bacteria treated with AgNPs.
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