Journal
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
Volume 45, Issue 2, Pages 340-348Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/21691401.2016.1153482
Keywords
Amperometry; biosensor; Co3O4; glutamate; graphene
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Funding
- Ankara University Research Fund [13L4240002]
- Scientific and Technological Research Council of Turkey
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An amperometric biosensor based on tricobalt tetraoxide nanoparticles (Co3O4), graphene (GR), and chitosan (CS) nanocomposite modified glassy carbon electrode (GCE) for sensitive determination of glutamate was fabricated. Scanning electron microscopy was implemented to characterize morphology of the nanocomposite. The biosensor showed optimum response within 25 s at pH 7.5 and 37 degrees C, at +0.70 V. The linear working range of biosensor for glutamate was from 4.0 x10(-6) to 6.0 x 10(-4) M with a detection limit of 2.0 x10(-6) M and sensitivity of 0.73 mu A/mM or 7.37 mu A/mMcm(2). The relatively low Michaelis-Menten constant (1.09 mM) suggested enhanced enzyme affinity to glutamate. The glutamate biosensor lost 45% of its initial activity after three weeks.
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