Journal
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume 16, Issue -, Pages 12-19Publisher
ELSEVIER
DOI: 10.1016/j.algal.2016.02.032
Keywords
Chlorella; CO2; Growth; Carbon concentrating mechanism; Gene expression
Categories
Funding
- National Natural Science Foundation of China [31300295, 21306222]
- Shanghai Education Development Foundation
- Shanghai Municipal Education Commission [14CG27]
- National Special Fund for State Key Laboratory of Bioreactor Engineering [2060204]
- National Basic Research Program of China [2011CB200904]
- Fundamental Research Funds for the Central Universities [WF1314033]
- Open Funding Project of the State Key Laboratory of Bioreactor Engineering [2060204]
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Microalgae can tolerate different CO2 concentrations and its mechanism of rapid CO2 fixation is key to understanding the relationship between intracellular substance and energy conversion, as well as to optimize the production of microalgae bioproducts. The present study conducted a transcriptome-based analysis of gene expression of an industrial Chlorella pyrenoidosa strain, FACHB-9, with high oil production ability under different CO2 concentrations. Transcriptome-based gene expression analysis indicated the redirection of central metabolism under CO2 deprivation. The C-3 pathway served as the main metabolic pathway for C. pyrenoidosa, which was subjected to a high CO2 environment in the present study. Similar to C-4 plants wherein limited CO2 activates CO2-concentrating mechanism to compensate for the low activity of RuBisCO in the Calvin cycle, C. pyrenoidosa undergoes CO2 compensation by active transport to ensure sufficient amounts of Ci for its growth and metabolism. Comparative analysis has allowed the identification of several candidate genes for further strain improvement. These genes encode proteins that might be CAs, as indicated by its localization to the cell membrane or chloroplast membrane, or act as Ci transporters that assist Ci transmembrane transportation. Certain types of ABC transport proteins and CCCH-type zinc finger proteins also showed significant changes in gene expression. These findings indicate that these may be promising targets for functional and genetic modification studies. The results not only reveal the significance of the mechanism of carbon sequestration to eukaryotic green algae, but also provide a basis for further construction of the energy microalgae cell factory with high efficiency carbon biofixation capacity. (C) 2016 Elsevier B.V. All rights reserved.
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