Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume -, Issue -, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.202311476
Keywords
Biosensors; Imaging Agents; Single-Walled Carbon Nanotubes; Glucose; Glucose Oxidase
Categories
Ask authors/readers for more resources
This study presents the development of near-infrared fluorescent single-walled carbon nanotube (SWCNT) based nanosensors for glucose imaging. The authors find that the fluorescence modulation of GOx-SWCNT is not associated with the enzymatic activity but rather triggered by the substrate-enzyme binding. Leveraging this unique mechanism, the authors developed catalytically inactive nanosensors that enable glucose imaging in mouse brain slices.
Continuous and non-invasive glucose monitoring and imaging is important for disease diagnosis, treatment, and management. However, glucose monitoring remains a technical challenge owing to the dearth of tissue-transparent glucose sensors. In this study, we present the development of near-infrared fluorescent single-walled carbon nanotube (SWCNT) based nanosensors directly functionalized with glucose oxidase (GOx) capable of immediate and reversible glucose imaging in biological fluids and tissues. We prepared GOx-SWCNT nanosensors by facile sonication of SWCNT with GOx in a manner that-surprisingly-does not compromise the ability of GOx to detect glucose. Importantly, we find by using denatured GOx that the fluorescence modulation of GOx-SWCNT is not associated with the catalytic oxidation of glucose but rather triggered by glucose-GOx binding. Leveraging the unique response mechanism of GOx-SWCNT nanosensors, we developed catalytically inactive apo-GOx-SWCNT that enables both sensitive and reversible glucose imaging, exhibiting a Delta F/F0 of up to 40 % within 1 s of exposure to glucose without consuming the glucose analyte. We finally demonstrate the potential applicability of apo-GOx-SWCNT in biomedical applications by glucose quantification in human plasma and glucose imaging in mouse brain slices. This paper presents the development of nIR fluorescent single-walled carbon nanotube (SWCNT) based nanosensors for glucose imaging. The authors find that the fluorescence modulation of GOx-SWCNT is not associated with the enzymatic activity but rather triggered by the substrate-enzyme binding. Leveraging this unique mechanism, the authors developed catalytically inactive nanosensors that enable glucose imaging in mouse brain slices.image
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available