Time-lapse observation of mouse preimplantation embryos using a simple closed glass capillary method

Time-lapse observation is a popular method for analyzing mammalian preimplantation embryos, but it often requires expensive equipment and skilled techniques. We previously developed a simply and costly embryo-culture system in a sealed tube that does not require a CO2 incubator. In the present study, we developed a new time-lapse observation system using our previous culture method and a glass capillary. Zygotes were placed in a glass capillary and sunk in oil for observation under a stereomicroscope. Warming the capillary using a thermoplate enabled most of the zygotes to develop into blastocysts and produce healthy offspring. This time-lapse observation system captured images every 30 min for up to 5 days, which confirmed that the developmental speed and quality of the embryos were not affected, even with fluorescence. Overall, this new system is a simple time-lapse observation method for preimplantation embryos that does not require dedicated machines and advanced techniques.

Automated summary

A new time-lapse observation system for preimplantation embryos was developed in this study, using a simple culture method and a glass capillary. The system allows the observation of mammalian preimplantation embryos without the need for expensive equipment and advanced techniques. The results confirmed that the developmental speed and quality of the embryos were not affected.