A chromosome-level genome assembly for the smoky rubyspot damselfly (Hetaerina titia)

Article Biotechnology & Applied Microbiology

HiFiAdapterFilt, a memory efficient read processing pipeline, prevents occurrence of adapter sequence in PacBio HiFi reads and their negative impacts on genome assembly

Sheina B. Sim et al.

Summary: Analysis of publicly available HiFi datasets showed the necessity of a read-sanitation step to remove sequence artifacts from PacBio library preparation to prevent adapter contamination in assemblies. The study presented HiFiAdapterFilt as a simple and memory efficient solution for this issue.

BMC GENOMICS (2022)

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Article Biotechnology & Applied Microbiology

Haplotype-resolved assembly of diploid genomes without parental data

Haoyu Cheng et al.

Summary: This paper presents an algorithm that combines PacBio HiFi reads and Hi-C chromatin interaction data to achieve haplotype-resolved genome assembly from single samples without the need for parent sequencing. The algorithm outperforms existing single-sample assembly pipelines and produces assemblies of similar quality to the best pedigree-based assemblies when applied to human and other vertebrate samples.

NATURE BIOTECHNOLOGY (2022)

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Article Biodiversity Conservation

Evolution and Biogeographic History of Rubyspot Damselflies (Hetaerininae: Calopterygidae: Odonata)

Samantha Standring et al.

Summary: In this study, a phylogenetic hypothesis was generated to test the relationships and divergence times within Hetaerininae. It was found that Mnesarete and Ormenophlebia render Hetaerina paraphyletic. The forest habitat was identified as the ancestral state for Hetaerininae, with transitions to generalist species primarily in the Mesoamerican region. The closure of the Central American Seaway was supported to have occurred gradually, starting in the Oligocene and ending in the Pliocene.

DIVERSITY-BASEL (2022)

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Article Biochemical Research Methods

Haplotype-resolved de novo assembly using phased assembly graphs with hifiasm

Haoyu Cheng et al.

Summary: hifiasm is a novel assembler that utilizes long high-fidelity sequence reads to accurately represent haplotype information, outperforming existing tools in haplotype-resolved assembly on various datasets, including a hexaploid genome dataset.

NATURE METHODS (2021)

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Article Multidisciplinary Sciences