Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand

Molecular forces generated by cell receptors are infrequent and transient, and hence difficult to detect. Here we report an assay that leverages the CRISPR-associated protein 12a (Cas12a) to amplify the detection of cellular traction forces generated by as few as 50 adherent cells. The assay involves the immobilization of a DNA duplex modified with a ligand specific for a cell receptor. Traction forces of tens of piconewtons trigger the dehybridization of the duplex, exposing a cryptic Cas12-activating strand that sets off the indiscriminate Cas12-mediated cleavage of a fluorogenic reporter strand. We used the assay to perform hundreds of force measurements using human platelets from a single blood draw to extract individualized dose-response curves and half-maximal inhibitory concentrations for a panel of antiplatelet drugs. For seven patients who had undergone cardiopulmonary bypass, platelet dysfunction strongly correlated with the need for platelet transfusion to limit bleeding. The Cas12a-mediated detection of cellular traction forces may be used to assess cell state, and to screen for genes, cell-adhesion ligands, drugs or metabolites that modulate cell mechanics. A fluorescence-based assay that leverages the enzyme Cas12a to transduce and amplify cumulative forces generated by cells allows for the quantification of platelet dysfunction, as shown with patients who had undergone cardiopulmonary bypass.

Automated summary

A detection method utilizing Cas12a has been reported to amplify cellular traction forces and evaluate platelet dysfunction. This method enables the extraction of individualized dose-response curves for antiplatelet drugs and the screening of genes, cell-adhesion ligands, drugs, or metabolites that modulate cell mechanics.