Journal
BIOMOLECULES
Volume 13, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/biom13111635
Keywords
docosahexaenoic acid; arachidonic acid; lipid signaling; membrane phospholipid; inflammation; monocytes/macrophages
Categories
Ask authors/readers for more resources
This study investigates the incorporation and redistribution of DHA in mouse macrophages. DHA is initially taken up by choline glycerophospholipids and transferred to ethanolamine glycerophospholipids through prolonged incubation. Mass spectrometry analysis reveals a high abundance of DHA in ether phospholipids. Stimulation of macrophages leads to decreases in DHA-containing PC and PI species, while an unusual DHA-containing species, PI(20:4/22:6), markedly increases.
In this work, the incorporation of docosahexaenoic acid (DHA) in mouse resident peritoneal macrophages and its redistribution within the various phospholipid classes were investigated. Choline glycerophospholipids (PC) behaved as the major initial acceptors of DHA. Prolonged incubation with the fatty acid resulted in the transfer of DHA from PC to ethanolamine glycerophospholipids (PE), reflecting phospholipid remodeling. This process resulted in the cells containing similar amounts of DHA in PC and PE in the resting state. Mass spectrometry-based lipidomic analyses of phospholipid molecular species indicated a marked abundance of DHA in ether phospholipids. Stimulation of the macrophages with yeast-derived zymosan resulted in significant decreases in the levels of all DHA-containing PC and PI species; however, no PE or PS molecular species were found to decrease. In contrast, the levels of an unusual DHA-containing species, namely PI(20:4/22:6), which was barely present in resting cells, were found to markedly increase under zymosan stimulation. The levels of this phospholipid also significantly increased when the calcium-ionophore A23187 or platelet-activating factor were used instead of zymosan to stimulate the macrophages. The study of the route involved in the synthesis of PI(20:4/22:6) suggested that this species is produced through deacylation/reacylation reactions. These results define the increases in PI(20:4/22:6) as a novel lipid metabolic marker of mouse macrophage activation, and provide novel information to understand the regulation of phospholipid fatty acid turnover in activated macrophages.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available