Pithoviruses Are Invaded by Repeats That Contribute to Their Evolution and Divergence from Cedratviruses

Pithoviridae are amoeba-infecting giant viruses possessing the largest viral particles known so far. Since the discovery of Pithovirus sibericum, recovered from a 30,000-yr-old permafrost sample, other pithoviruses, and related cedratviruses, were isolated from various terrestrial and aquatic samples. Here, we report the isolation and genome sequencing of 2 Pithoviridae from soil samples, in addition to 3 other recent isolates. Using the 12 available genome sequences, we conducted a thorough comparative genomic study of the Pithoviridae family to decipher the organization and evolution of their genomes. Our study reveals a nonuniform genome organization in 2 main regions: 1 concentrating core genes and another gene duplications. We also found that Pithoviridae genomes are more conservative than other families of giant viruses, with a low and stable proportion (5% to 7%) of genes originating from horizontal transfers. Genome size variation within the family is mainly due to variations in gene duplication rates (from 14% to 28%) and massive invasion by inverted repeats. While these repeated elements are absent from cedratviruses, repeat-rich regions cover as much as a quarter of the pithoviruses genomes. These regions, identified using a dedicated pipeline, are hotspots of mutations, gene capture events, and genomic rearrangements that contribute to their evolution.

Automated summary

This study reports the isolation and genome sequencing of two Pithoviridae viruses from soil samples, as well as three other recent isolates. A comparative genomic study of the Pithoviridae family using twelve available genome sequences reveals a nonuniform genome organization, with core genes concentrated in one region and gene duplications in another. The study also shows that Pithoviridae genomes are more conservative than other families of giant viruses, with a low proportion of genes originating from horizontal transfers. Genome size variation within the family is mainly due to variations in gene duplication rates and massive invasion by inverted repeats.