4.7 Review

Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review

Related references

Note: Only part of the references are listed.
Article Biotechnology & Applied Microbiology

A novel paper based loop mediated isothermal amplification and lateral flow assay (LAMP-LFA) for point-of-care detection of buffalo tissue origin in diverse foods

Jyoti Jawla et al.

Summary: In this study, a paper based loop-mediated isothermal amplification-lateral flow (LAMP-LF) Assay was developed for on-site detection of buffalo tissue. The assay demonstrated high specificity and low detection limit. The efficiency and accuracy of the assay were validated using different types of meat samples and compared with conventional PCR outcomes. The paper platform also showed potential for deployment in low-resource settings.

JOURNAL OF FOOD SAFETY (2023)

Article Chemistry, Analytical

Electrolysis of Bacteria Based on Microfluidic Technology

Jianqiu Zhao et al.

Summary: Cell lysis is an important step for protein research and various applications such as drug screening and cancer diagnosis. Current cell lysis methods have limitations, prompting the need for a fast, chemical-free, portable, and non-invasive device. In this study, an integrated microfluidic chip was designed to achieve E. coli lysis using an alternating current electric field, and the effects of voltage, frequency, and flow rate on lysis efficiency were investigated. The study provided a simple and efficient method for bacteria lysis in biology and biomedical applications.

MICROMACHINES (2023)

Article Materials Science, Multidisciplinary

Paper-Based Bacterial Lysis Enables Sample-to-Answer Home-based DNA Testing

Georgios Chondrogiannis et al.

Summary: Nucleic acid amplification testing (NAAT) is the gold standard for infectious disease diagnostics, but it is mainly limited to centralized laboratories. Researchers have addressed the bottleneck issue of DNA extraction by immobilizing the enzyme ACP on nitrocellulose, enabling home-based kits and expanding the capabilities of home-testing beyond antigen tests.

ADVANCED MATERIALS TECHNOLOGIES (2023)

Article Biochemical Research Methods

A novel paper-based lysis strip for SARS-CoV-2 RNA detection at low resource settings

Songhyun Lee et al.

Summary: Infectious respiratory diseases like COVID-19 are a serious global concern. A simple and reliable molecular diagnosis platform for Point of Care Test (POCT) is required to control the spread in low-resource settings. We propose a paper-based lysis (PBL) strip for nucleic acid extraction, which effectively isolates RNA from viruses with biological interference and inhibitors. By optimizing the buffer compositions and membranes of the strip, a prepared eluent can be obtained within 20 minutes for downstream inspection, detecting 104 copies/swabs within 20 minutes when combined with Reverse Transcription Loop-Mediated Amplification (RT-LAMP).

ANALYTICAL BIOCHEMISTRY (2023)

Article Multidisciplinary Sciences

Disposable platform for bacterial lysis and nucleic acid amplification based on a single USB-powered printed circuit board

Kamal G. Shah et al.

Summary: Recent advances in electronics and microfluidics have enabled the development of integrated isothermal nucleic acid amplification test (NAAT) platforms for low-resource settings, such as homes. This study presents a generalizable strategy to integrate all heaters and control electronics onto a single low-cost, USB-powered printed circuit board, demonstrating the feasibility of translating NAATs to the home.

PLOS ONE (2023)

Article Acoustics

Inactivation of the enveloped virus phi6 with hydrodynamic cavitation

Mojca Zupanc et al.

Summary: The study investigates the effects of hydrodynamic cavitation on the inactivation of SARS-CoV-2 surrogate bacteriophage phi6. The results show that hydrodynamic cavitation can significantly reduce the virus count at certain temperatures. This suggests that hydrodynamic cavitation has the potential to inactivate current and potential enveloped viruses in water at lower temperatures.

ULTRASONICS SONOCHEMISTRY (2023)

Review Chemistry, Multidisciplinary

Toward Rapid and Accurate Molecular Diagnostics at Home

Wenpeng Liu et al.

Summary: The global outbreaks of infectious diseases have led to a significant demand for rapid and accurate molecular diagnostics. However, the labor-intensive sample preparation and nucleic acid amplification steps make it challenging to carry out these diagnostics at home. This review discusses the advances and challenges in automatic solutions for sample preparation and on-chip nucleic acid amplification, as well as the potential for game-changing user-friendly approaches for next-generation home diagnostics.

ADVANCED MATERIALS (2023)

Review Chemistry, Analytical

Paper-Based Loop Mediated Isothermal Amplification (LAMP) Platforms: Integrating the Versatility of Paper Microfluidics with Accuracy of Nucleic Acid Amplification Tests

Debayan Das et al.

Summary: Paper-based diagnostics offer a cost-effective and portable alternative to traditional diagnostic methods. However, current paper-based serology tests lack the accuracy and sensitivity of molecular diagnostic tests. Loop-mediated isothermal amplification (LAMP) provides PCR-like performance while simplifying the assay complexity. This review discusses the integration of paper microfluidics with LAMP for improved accuracy and versatility in point-of-care testing.

CHEMOSENSORS (2023)

Review Chemistry, Analytical

Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

Jocelyn Reynolds et al.

Summary: Isothermal nucleic acid amplification tests, which only require a constant temperature and simplify the amplification process, have gained popularity over PCR. Paper microfluidics have been extensively used for automated nucleic acid extraction, amplification, and detection in these isothermal amplification tests.

BIOSENSORS-BASEL (2023)

Article Chemistry, Analytical

A modular and reconfigurable open-channel gated device for the electrokinetic extraction of cell-free DNA assays

Nobuyuki Futai et al.

Summary: This study presents a method for the high-efficiency separation and extraction of short cfDNA fragments, using a millimeter-scale fluidic device to extract the fragments into solid agarose gel slabs from liquid human plasma samples. The method demonstrates high sensitivity in cfDNA detection.

ANALYTICA CHIMICA ACTA (2022)

Article Engineering, Biomedical

Functional hydrogel for fast, precise and inhibition-free point-of-care bacteria analysis in crude food samples

Xingyu Lin et al.

Summary: A nanoporous hydrogel with controlled release function was developed for nucleic acid analysis in crude food samples, allowing rapid, precise, and inhibition-free amplification. The hydrogel's nanoporous structures possess various abilities such as adsorption, release, separation, restriction, and self-cleaning, facilitating the amplification process. This novel lab-on-hydrogel concept holds great potential for future molecular diagnostic assays and point-of-care applications.

BIOMATERIALS (2022)

Article Chemistry, Analytical

Lateral flow assays (LFA) for detection of pathogenic bacteria: A small point-of-care platform for diagnosis of human infectious diseases

Hessamaddin Sohrabi et al.

Summary: Point-of-care testing (POCT) analysis and bedside research have globally improved up-to-date diagnostics. Lateral flow assays (LFAs) have gained considerable attention due to their cost-effectiveness, portability, user-friendliness, and rapid detection. LFAs based on qualitative and optical outcomes have enhanced the objectivity and data efficiency of assessments. Therefore, developing novel methods to provide reliable and quantitative information while preserving the qualities of LFAs is of great necessity.

TALANTA (2022)

Review Chemistry, Analytical

REASSURED Multiplex Diagnostics: A Critical Review and Forecast

Jonas A. Otoo et al.

Summary: This review summarizes current diagnostic technologies for multiplexed detection and explores which methods are likely to meet all the REASSURED criteria.

BIOSENSORS-BASEL (2022)

Article Biophysics

A finger-driven disposable micro-platform based on isothermal amplification for the application of multiplexed and point-of-care diagnosis of tuberculosis

Zhiying Wang et al.

Summary: A low-cost, portable finger-driven microfluidic chip was developed for rapid on-site detection of tuberculosis, showing unique advantages in avoiding risks of infection or environmental contamination and enabling multiplexed detection to differentiate different strains of Mycobacterium. In clinical trials, the platform demonstrated high specificity and sensitivity.

BIOSENSORS & BIOELECTRONICS (2022)

Article Biochemical Research Methods

Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids

Miranda N. Emaus et al.

Summary: In this study, a fast and convenient method for plant DNA sample preparation using hydrophobic magnetic ionic liquids and ionic liquids was developed. This method does not require high temperatures or chemical surfactants and can be completed in one minute or less, making it suitable for fieldwork and lab work in low resource environments.

PLANT METHODS (2022)

Article Acoustics

Cavitation bubble interaction with compliant structures on a microscale: A contribution to the understanding of bacterial cell lysis by cavitation treatment

Jure Zevnik et al.

Summary: This paper investigates the interaction between a single bubble and a structure resembling a bacterial cell to elucidate the process of cavitation-assisted water treatment. The results show that bubble-induced local stresses can damage bacterial cell membranes, with bacterial cell damage primarily caused by mechanical effects rather than thermal and chemical ones.

ULTRASONICS SONOCHEMISTRY (2022)

Article Virology

Low-Cost and Rapid Method of DNA Extraction from Scaled Fish Blood and Skin Mucus

Lang Gui et al.

Summary: This study presents a simple, inexpensive, and efficient method for extracting DNA samples from fish using swabbing and disc protocol. DNA can be extracted from fish mucus for species identification and viral pathogen detection. This method can be applied in aquafarms and aqua stores outside the laboratory environment.

VIRUSES-BASEL (2022)

Review Biotechnology & Applied Microbiology

New horizons in developing cell lysis methods: A review

Mohsen Danaeifar

Summary: Cell lysis is a crucial step in biology and medicine research, with various types of methods available. Traditional approaches and new techniques are being developed to provide more precise and efficient tools while reducing the costs. However, challenges and improvements still remain.

BIOTECHNOLOGY AND BIOENGINEERING (2022)

Review Chemistry, Analytical

Lateral flow assays for viruses diagnosis: Up-to-date technology and future prospects

Bahar Ince et al.

Summary: This article introduces the application of portable, disposable, and inexpensive lateral flow assay (LFA) systems in the diagnosis of infectious diseases. It first examines the design principles and working mechanisms of existing LFA methods and then presents current LFA implementation strategies for diseases such as COVID-19, Zika and dengue, HIV, hepatitis, influenza, malaria, and others. Furthermore, it summarizes the current problems and accessible solutions in detecting infectious agents and diseases by LFA, with a focus on increasing sensitivity through various detection methods. The article also envisions future trends in LFA-based diagnostics.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2022)

Article Chemistry, Applied

Alkaline lysis-recombinase polymerase amplification combined with CRISPR/Cas12a assay for the ultrafast visual identification of pork in meat products

Gang Zhao et al.

Summary: The study successfully established a sensitive and rapid detection method for pig-derived components using CRISPR/Cas12a combined with recombinase polymerase amplification. The researchers obtained satisfactory results by testing beef and pork binary mixture models under different processing conditions, as well as combining with two different DNA extraction methods.

FOOD CHEMISTRY (2022)

Article Biochemistry & Molecular Biology

Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional Crosslinkers

HyeonAh Seong et al.

Summary: Cell-free DNA (cfDNA) has been widely studied in cancer management using liquid biopsy. In this study, we developed a magnetic bead-based cfDNA extraction method using homobifunctional crosslinkers, which resulted in higher extraction efficiency and shorter processing time.

BIOMEDICINES (2022)

Article Biochemistry & Molecular Biology

Nucleic acid extraction from complex biofluid using toothpick-actuated over-the-counter medical-grade cotton

Shrawan Kumar et al.

Summary: Nucleic acid amplification technique (NAAT)-assisted detection is crucial for pathogen molecular diagnostics, but prior nucleic acid extraction is required. Traditional extraction methods limit the use of qPCR or PCR-like NAAT methods in resource-constrained settings.

BIOORGANIC & MEDICINAL CHEMISTRY (2022)

Review Chemistry, Analytical

Isothermal Amplification Technology for Disease Diagnosis

Poramin Boonbanjong et al.

Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.

BIOSENSORS-BASEL (2022)

Article Chemistry, Analytical

A sample-to-answer COVID-19 diagnostic device based on immiscible filtration and CRISPR-Cas12a-assisted detection

Bongkot Ngamsom et al.

Summary: In response to the disparities of vaccination coverage in low-and middle-income countries, this study presents a lab-on-a-chip device called 'IFAST-LAMP-CRISPR', which provides an affordable, rapid, and high-precision molecular diagnostic means for detecting SARS-CoV-2. The device integrates RNA extraction, amplification, molecular detection, and lateral flow readout in one device, offering a simple, cost-effective, and integrated platform for COVID-19 diagnostics in low-resource settings.

TALANTA OPEN (2022)

Article Biochemical Research Methods

Homebrew: An economical and sensitive glassmilk-based nucleic-acid extraction method for SARS-CoV-2 diagnostics

Robert Page et al.

Summary: This study establishes a low-cost method for detecting SARS-CoV-2, which allows for automation in high-throughput settings. The method offers similar sensitivity to commercial kits but requires less time and reagents, and also enables sample pooling without loss of sensitivity. This method has the potential to facilitate more economical widespread testing, particularly in resource-limited settings.

CELL REPORTS METHODS (2022)

Article Nanoscience & Nanotechnology

Postsynthetic Modification of the Magnetic Zirconium-Organic Framework for Efficient and Rapid Solid-Phase Extraction of DNA

Wang Pan et al.

Summary: In this study, Zr-based MOFs for DNA extraction were successfully synthesized and functionalized with Fe3O4 nanoparticles to introduce magnetism. The magnetic zirconium-organic framework showed high efficiency in DNA recovery studies, especially demonstrating superior performance in extracting DNA from diverse sample types.

ACS APPLIED MATERIALS & INTERFACES (2021)

Article Biochemical Research Methods

Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis

Benjamin P. Sullivan et al.

Summary: The translation introduces a paper-based device that uses isotachophoresis to extract and purify nucleic acids from whole blood, providing a simpler sample preparation method for point-of-care NAATs.

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2021)

Article Biochemical Research Methods

Improved high-molecular-weight DNA extraction, nanopore sequencing and metagenomic assembly from the human gut microbiome

Dylan G. Maghini et al.

Summary: Utilizing a combination of short-read metagenomic sequencing and long-read sequencing, high-quality bacterial genomes can be obtained from the complex human gut microbiome, with some challenges to overcome.

NATURE PROTOCOLS (2021)

Article Food Science & Technology

Ultrafast bacterial cell lysis using a handheld corona treater and loop-mediated isothermal amplification for rapid detection of foodborne pathogens

Mengzhe Li et al.

Summary: The study introduced an ultrafast bacterial cell lysis method induced by corona discharge, combined with loop-mediated isothermal amplification (LAMP) for rapid and convenient detection of foodborne pathogens. The corona discharge effectively destabilized bacterial cell envelopes and released more DNA, providing shorter preparation time and higher DNA yields compared to thermal lysis. Collaborating with colorimetric LAMP, the platform allowed visual detection of bacterial DNA in a time-efficient manner.

FOOD CONTROL (2021)

Article Chemistry, Analytical

Point-of-care DNA testing by automatically and sequentially performing extraction, amplification and identification in a closed-type cassette

Tianhui Dong et al.

Summary: This study presents a fully automatic and contamination-free cassette system for nucleic acid detection, which can detect up to 10 targets in a sample with high accuracy and simplicity. The closed round design of the system allows for effective point-of-care genetic testing.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

Article Chemistry, Multidisciplinary

Fluorometric Paper-Based, Loop-Mediated Isothermal Amplification Devices for Quantitative Point-of-Care Detection of Methicillin-Resistant Staphylococcus aureus (MRSA)

Ilada Choopara et al.

Summary: The research team developed a fluorometric paper-based LAMP technology for rapid MRSA detection. By optimizing reaction conditions, they created a paper-based MRSA diagnostic device that can accurately detect low levels of MRSA in a short amount of time, demonstrating high specificity and sensitivity in both patient and laboratory samples.

ACS SENSORS (2021)

Article Chemistry, Analytical

Paper-based loop-mediated isothermal amplification and lateral flow (LAMP-LF) assay for identification of tissues of cattle origin

Jyoti Jawla et al.

Summary: The study developed a rapid detection method for identifying cattle tissue with high specificity and analytical sensitivity. Laboratory validation and comparison with PCR detection methods demonstrated the accuracy of the developed assay. Additionally, the assay was shown to provide rapid results in field applications.

ANALYTICA CHIMICA ACTA (2021)

Article Biochemical Research Methods

Spherical Nucleic Acid Mediated Functionalization of Polydopamine-Coated Nanoparticles for Selective DNA Extraction and Detection

Mohamad Zandieh et al.

Summary: In this study, a core-shell magnetic structure coated with polydopamine was designed to achieve highly selective DNA extraction by functionalizing with spherical nucleic acids. The findings suggest that SNA allows for a higher probe density on the NPs compared to SH-DNA, leading to faster and more effective DNA extraction. This surface modification strategy using PDA@SNA conjugates provides a general approach for sequence-specific DNA extraction.

BIOCONJUGATE CHEMISTRY (2021)

Article Multidisciplinary Sciences

Extraction-free protocol combining proteinase K and heat inactivation for detection of SARS-CoV-2 by RT-qPCR

Valeria Genoud et al.

Summary: The PK+HID method is an extraction-free RT-qPCR technique for SARS-CoV-2 detection, showing improved performance compared to traditional heat-inactivation procedures. It can be combined with multiplexing RT-qPCR kits, demonstrating high sensitivity and specificity in clinical sample analysis.

PLOS ONE (2021)

Article Chemistry, Analytical

Cavitation-microstreaming-based lysis and DNA extraction using a laser-machined polycarbonate microfluidic chip

Abdi Mirgissa Kaba et al.

Summary: In this study, a microfluidic cavitation-microstreaming-based cell lysis and DNA extraction method was introduced, which showed efficient lysis and extraction performance. The rapid fabrication of the microfluidic device and simple operation led to a short DNA extraction time and high extraction efficiency.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

Article Ecology

On-site genetic analysis for species identification using lab-on-a-chip

Ryan Wimbles et al.

Summary: This study developed a microfluidic device for genetic analysis of White Rhinoceros dung samples, providing a portable and cost-effective solution for species identification to aid conservation efforts. Optimization of DNA extraction processes and the use of specific amplification reactions allowed for accurate detection results to be obtained quickly.

ECOLOGY AND EVOLUTION (2021)

Article Chemistry, Analytical

A Sample-In-Answer-Out Microfluidic System for the Molecular Diagnostics of 24 HPV Genotypes Using Palm-Sized Cartridge

Rui Wang et al.

Summary: This research presents an automated microfluidic system that integrates the functions of a traditional PCR laboratory into a palm-sized microfluidic card, capable of typing 24 HPV genotypes. The system achieves a detection limit of 200 copies/test and a 100% positive detection rate for clinical samples, making it an effective method for HPV detection.

MICROMACHINES (2021)

Article Chemistry, Analytical

Multiplexed Centrifugal Microfluidic System for Dynamic Solid-Phase Purification of Polynucleic Acids Direct from Buccal Swabs

Leah M. Dignan et al.

Summary: This report describes the development of a centrifugally controlled microfluidic dynamic solid-phase extraction platform for obtaining amplification-ready nucleic acids directly from buccal swab cuttings. The technology showed broad utility by successfully establishing downstream compatibility with multiple nucleic acid assays. The automatable, portable microfluidic platform for nucleic acid preparation device could enable practical, in situ use by nontechnical personnel.

ANALYTICAL CHEMISTRY (2021)

Article Microbiology

Freeze-Thaw Pretreatment Can Improve Efficiency of Bacterial DNA Extraction From Meconium

Yuntian Xin et al.

Summary: This study aimed to improve the efficiency of bacterial DNA extraction from meconium, a challenging process due to low microflora levels and PCR inhibitors. Using freeze-thawing cycles and different freezing conditions, the researchers found that freezing at -20 degrees Celsius was the best condition for DNA extraction and preservation of microbial diversity in meconium. The developed protocol could significantly enhance DNA extraction efficiency and preserve the species diversity of meconium microflora, providing potential benefits for newborn bacterial colonization studies.

FRONTIERS IN MICROBIOLOGY (2021)

Article Biochemistry & Molecular Biology

High-Quality Nucleic Acid Isolation from Hard-to-Lyse Bacterial Strains Using PMAP-36, a Broad-Spectrum Antimicrobial Peptide

Hye-sun Cho et al.

Summary: The study found that using broad-spectrum antimicrobial peptide PMAP-36 can effectively isolate high-quality RNA from various bacteria, providing an efficient method for nucleic acid isolation. Additionally, the use of PMAP-36 also successfully isolates high-quality genomic DNA from challenging bacterial strains, offering a new option for microbiome research.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Review Nanoscience & Nanotechnology

Systematic review of centrifugal valving based on digital twin modeling towards highly integrated lab-on-a-disc systems

Jens Ducree

Summary: This review discusses the challenges and current trends in larger-scale integration of microfluidic systems, highlighting the advantages of centrifugal systems for sample preparation. It also introduces a method using simulation tools for virtual prototyping and design optimization.

MICROSYSTEMS & NANOENGINEERING (2021)

Article Chemistry, Analytical

Paper-Based Airborne Bacteria Collection and DNA Extraction Kit

Youngung Seok et al.

Summary: The paper-based airborne bacteria collection and DNA extraction kit developed in this study offers a simple, rapid, and cost-effective method for monitoring airborne pathogens. With the integration of 3D printing technology and an air pump, the entire operation process can be completed within 20 minutes, making it suitable for use in resource-limited settings. The effectiveness of the paper kit was demonstrated under specific conditions, showing potential for widespread application in monitoring airborne pathogens efficiently.

BIOSENSORS-BASEL (2021)

Review Chemistry, Analytical

Review of Microfluidic Methods for Cellular Lysis

Emil Grigorov et al.

Summary: Cell lysis is a crucial process to extract important information about DNA or RNA of an organism. This article provides a comprehensive review of various methods for effective cellular disintegration, categorized into six types. The advantages and disadvantages of each method are outlined, with a concise table comparing benefits based on criteria like cost, efficiency, and difficulty.

MICROMACHINES (2021)

Article Chemistry, Analytical

A lab-on-a-chip platform for integrated extraction and detection of SARS-CoV-2 RNA in resource-limited settings

Pablo Rodriguez-Mateos et al.

Summary: Efforts are needed to develop rapid and accurate diagnostic tools for extensive testing of COVID-19. Current gold standard nucleic acid tests require trained personnel and specialist instrumentation, which hampers turnaround time and test accessibility, especially in low-resource settings.

ANALYTICA CHIMICA ACTA (2021)

Article Biochemical Research Methods

An effective method for saliva stabilization and magnetic nanoparticles based DNA extraction for genomic applications

Aniruddha Bhati et al.

Summary: An improved method for DNA extraction from human saliva using uncoated magnetic nanoparticles is described, aiming to address the issues of low efficiency and storage of saliva samples. Additionally, a saliva preservation buffer was developed to ensure stability of saliva samples for up to 160 days at room temperature, with intact quality of genomic DNA.

ANALYTICAL BIOCHEMISTRY (2021)

Article Biophysics

Nanoporous hydrogel for direct digital nucleic acid amplification in untreated complex matrices for single bacteria counting

Changyu Yi et al.

Summary: A nanoporous polyethylene glycol hydrogel with self-cleaning capacity was developed for direct amplification of nucleic acid in complex matrices without sample pretreatment. The hydrogel facilitated nucleic acid amplification by adsorbing and removing inhibitors during isothermal amplification, resulting in successful amplification even in the presence of inhibitors. Digital quantification of pathogens in whole blood was achieved within 20 minutes, demonstrating high specificity and low detection limit.

BIOSENSORS & BIOELECTRONICS (2021)

Article Biophysics

An integrated E-Tube cap for sample preparation, isothermal amplification and label-free electrochemical detection of DNA

Zhiheng Xu et al.

Summary: A simple, disposable, and integrated electronic-tube cap (E-tube cap) for DNA detection at the point-of-care was developed and successfully demonstrated the presence of 100 copies of λDNA in saliva samples. The device showed fast, accurate, and cost-effective diagnostic capabilities, making it particularly suitable for point-of-care molecular diagnostics of infectious diseases.

BIOSENSORS & BIOELECTRONICS (2021)

Article Multidisciplinary Sciences

Oil immersed lossless total analysis system for integrated RNA extraction and detection of SARS-CoV-2

Duane S. Juang et al.

Summary: The authors report the development of OIL-TAS, a system that integrates RNA extraction and detection into a single device, which shows promising performance in clinical sample testing with the potential to offer a faster, cheaper, and easier alternative for infectious disease testing.

NATURE COMMUNICATIONS (2021)

Article Biotechnology & Applied Microbiology

Improvements in Extraction Methods of High-molecular-weight DNA from Soils by Modifying Cell Lysis Conditions and Reducing Adsorption of DNA onto Soil Particles

Yoriko Sakai

Summary: Improving extraction procedures for HMW DNA from soil samples can increase the size and yield of extracted DNA. In Andosols soil group, the addition of boiled sonicated salmon DNA was found to be effective for HMW DNA extraction.

MICROBES AND ENVIRONMENTS (2021)

Article Plant Sciences

Rapid and simple detection of Phytophthora cactorum in strawberry using a coupled recombinase polymerase amplification-lateral flow strip assay

Xinyu Lu et al.

Summary: A lateral flow recombinase polymerase amplification (LF-RPA) assay was developed for sensitive visual detection of Phytophthora cactorum, with a detection limit of 100 fg of genomic DNA. The assay successfully detected P. cactorum in naturally diseased strawberry samples within 40 minutes, making it a rapid, simple, and accurate method for detection with potential for application in resource-limited laboratories.

PHYTOPATHOLOGY RESEARCH (2021)

Article Chemistry, Analytical

HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification

Andrew T. Bender et al.

Summary: The current total of people living with HIV is close to 38 million, with about 26 million on antiretroviral therapy. Efforts to expand access to HIV nucleic acid testing in low- and middle-income countries have been hindered by the cost and complexity of current tests. Traditional RNA extraction methods remain a key bottleneck for developing low-cost POC nucleic acid tests.

ANALYST (2021)

Article Nanoscience & Nanotechnology

Low-Resource Nucleic Acid Extraction Method Enabled by High-Gradient Magnetic Separation

Stephanie Pearlman et al.

ACS APPLIED MATERIALS & INTERFACES (2020)

Article Engineering, Biomedical

Poly-L-histidine coated microfluidic devices for bacterial DNA purification without chaotropic solutions

Athina S. Kastania et al.

BIOMEDICAL MICRODEVICES (2020)

Article Chemistry, Applied

An innovative paper-based device for DNA extraction from processed meat products

Bhagwan S. Batule et al.

FOOD CHEMISTRY (2020)

Article Biochemical Research Methods

Simultaneous cell lysis and DNA extraction from whole blood using magnetic ionic liquids

Miranda N. Emaus et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020)

Review Chemistry, Analytical

Magnetic particles for integrated nucleic acid purification, amplification and detection without pipetting

Yanju Chen et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2020)

Review Chemistry, Analytical

Nucleic acid extraction: Fundamentals of sample preparation methodologies, current advancements, and future endeavors

Miranda N. Emaus et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2020)

Article Biochemical Research Methods

Magnetic particle transport through organogel - an application to DNA extraction

Tetsuo Ohashi et al.

ANALYTICAL BIOCHEMISTRY (2020)

Article Materials Science, Multidisciplinary

Hydrogen bonding interactions of H2O and SiOH on a boroaluminosilicate glass corroded in aqueous solution

Dien Ngo et al.

NPJ MATERIALS DEGRADATION (2020)

Article Chemistry, Analytical

Synergistic use of electroosmotic flow and magnetic forces for nucleic acid extraction

Rachel N. Deraney et al.

ANALYST (2020)

Article Biochemical Research Methods

A sample-in-multiplex-digital-answer-out chip for fast detection of pathogens

Juxin Yin et al.

LAB ON A CHIP (2020)

Article Biochemical Research Methods

A point of care platform based on microfluidic chip for nucleic acid extraction in less than 1minute

Jianzhong Zhang et al.

BIOMICROFLUIDICS (2019)

Article Chemistry, Analytical

Centrifugation-Assisted Immiscible Fluid Filtration for Dual-Bioanalyte Extraction

Duane S. Juang et al.

ANALYTICAL CHEMISTRY (2019)

Article Biochemical Research Methods

Direct genotyping from whole blood using alkaline polyethylene glycol

Xiaonan Liu et al.

ANALYTICAL BIOCHEMISTRY (2019)

Review Biochemical Research Methods

Nip the bubble in the bud: a guide to avoid gas nucleation in microfluidics

Iago Pereiro et al.

LAB ON A CHIP (2019)

Article Biochemical Research Methods

Sample pre-concentration on a digital microfluidic platform for rapid AMR detection in urine

Sumit Kalsi et al.

LAB ON A CHIP (2019)

Article Chemistry, Analytical

Semiquantitative Nucleic Acid Test with Simultaneous Isotachophoretic Extraction and Amplification

Andrew T. Bender et al.

ANALYTICAL CHEMISTRY (2018)

Article Biochemistry & Molecular Biology

miFAST: A novel and rapid microRNA target capture method

Ashley M. Poenitzsch Strong et al.

MOLECULAR CARCINOGENESIS (2018)

Article Chemistry, Analytical

Rapid virus diagnostic system using bio-optical sensor and microfluidic sample processing

Choong Eun Jin et al.

SENSORS AND ACTUATORS B-CHEMICAL (2018)

Article Biochemical Research Methods

A sample-in-digital-answer-out system for rapid detection and quantitation of infectious pathogens in bodily fluids

Haowen Yang et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2018)

Article Biochemical Research Methods

An electro-conductive plane heating element for rapid thermal lysis of bacterial cells

YoungJoong Kim et al.

JOURNAL OF MICROBIOLOGICAL METHODS (2018)

Article Chemistry, Multidisciplinary

Integration of Nucleic Acid Extraction Protocol with Automated Extractor for Multiplex Viral Detection

Zeeshan Ali et al.

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY (2017)

Review Plant Sciences

Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection

Han Yih Lau et al.

FRONTIERS IN PLANT SCIENCE (2017)

Article Chemistry, Analytical

Rapid Nucleic Acid Extraction and Purification Using a Miniature Ultrasonic Technique

Darren W. Branch et al.

MICROMACHINES (2017)

Review Chemistry, Analytical

A Review on Macroscale and Microscale Cell Lysis Methods

Mohammed Shehadul Islam et al.

MICROMACHINES (2017)

Article Chemistry, Analytical

Use of Dimethyl Pimelimidate with Microfluidic System for Nucleic Acids Extraction without Electricity

Choong Eun Jin et al.

ANALYTICAL CHEMISTRY (2017)

Article Chemistry, Multidisciplinary

Facile silicification of plastic surface for bioassays

Seonki Hong et al.

CHEMICAL COMMUNICATIONS (2017)

Article Chemistry, Physical

Effect of surfactants on carryover liquid volume in immiscible phase magnetic bead separation

Derek Troiano et al.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS (2017)

Article Chemistry, Multidisciplinary

Effect of surfactant concentration on foam: From coreflood experiments to implicit-texture foam-model parameters

S. A. Jones et al.

JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY (2016)

Article Biochemical Research Methods

Comparison of point-of-care-compatible lysis methods for bacteria and viruses

Erin K. Heiniger et al.

JOURNAL OF MICROBIOLOGICAL METHODS (2016)

Article Biochemical Research Methods

Self-powered switch-controlled nucleic acid extraction system

Kyungsup Han et al.

LAB ON A CHIP (2016)

Article Biochemical Research Methods

An integrated paper-based sample-to-answer biosensor for nucleic acid testing at the point of care

Jane Ru Choi et al.

LAB ON A CHIP (2016)

Article Biochemical Research Methods

Virtual microfluidics for digital quantification and single-cell sequencing

Liyi Xu et al.

NATURE METHODS (2016)

Article Multidisciplinary Sciences

Isolating Influenza RNA from Clinical Samples Using Microfluidic Oil-Water Interfaces

Francis R. Cui et al.

PLOS ONE (2016)

Article Biotechnology & Applied Microbiology

Cell lysis induced by membrane-damaging detergent saponins from Quillaja saponaria

Joanna Berlowska et al.

ENZYME AND MICROBIAL TECHNOLOGY (2015)

Article Chemistry, Multidisciplinary

Design of low-cost ionic liquids for lignocellulosic biomass pretreatment

Anthe George et al.

GREEN CHEMISTRY (2015)

Article Materials Science, Multidisciplinary

Liquid carry-over in an injection moulded all-polymer chip system for immiscible phase magnetic bead-based solid-phase extraction

Kasper Kistrup et al.

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS (2015)

Article Multidisciplinary Sciences

An optimised direct lysis method for gene expression studies on low cell numbers

Anh Viet-Phuong Le et al.

SCIENTIFIC REPORTS (2015)

Article Pathology

HIV Viral RNA Extraction in Wax Immiscible Filtration Assisted by Surface Tension (IFAST) Devices

Scott M. Berry et al.

JOURNAL OF MOLECULAR DIAGNOSTICS (2014)

Review Biochemical Research Methods

Emerging microfluidic devices for cell lysis: a review

Lang Nan et al.

LAB ON A CHIP (2014)

Article Biochemistry & Molecular Biology

Protocols for metagenomic DNA extraction and Illumina amplicon library preparation for faecal and swab samples

A. -T. E. Vo et al.

MOLECULAR ECOLOGY RESOURCES (2014)

Article Chemistry, Multidisciplinary

Paper-based molecular diagnostic for Chlamydia trachomatis

Jacqueline C. Linnes et al.

RSC ADVANCES (2014)

Review Biophysics

The Mechanism of Detergent Solubilization of Lipid Bilayers

Dov Lichtenberg et al.

BIOPHYSICAL JOURNAL (2013)

Article Multidisciplinary Sciences

Impact of macromolecular crowding on DNA replication

Barak Akabayov et al.

NATURE COMMUNICATIONS (2013)

Article Biochemistry & Molecular Biology

Cost-effective, high-throughput DNA sequencing libraries for multiplexed target capture

Nadin Rohland et al.

GENOME RESEARCH (2012)

Review Biotechnology & Applied Microbiology

PCR inhibitors - occurrence, properties and removal

C. Schrader et al.

JOURNAL OF APPLIED MICROBIOLOGY (2012)

Article Microbiology

Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device

Peter E. Vandeventer et al.

JOURNAL OF CLINICAL MICROBIOLOGY (2011)

Review Biophysics

Nucleases: diversity of structure, function and mechanism

Wei Yang

QUARTERLY REVIEWS OF BIOPHYSICS (2011)

Article Chemistry, Physical

Dielectric Constants of Water, Methanol, Ethanol, Butanol and Acetone: Measurement and Computational Study

M. Mohsen-Nia et al.

JOURNAL OF SOLUTION CHEMISTRY (2010)

Article Chemistry, Analytical

Purification of Nucleic Acids from Whole Blood Using Isotachophoresis

Alexandre Persat et al.

ANALYTICAL CHEMISTRY (2009)

Article Biophysics

Common Crowding Agents Have Only a Small Effect on Protein-Protein Interactions

Yael Phillip et al.

BIOPHYSICAL JOURNAL (2009)

Review Biochemistry & Molecular Biology

Molecular crowding effects on structure and stability of DNA

Daisuke Miyoshi et al.

BIOCHIMIE (2008)

Article Chemistry, Analytical

Continuous flow microfluidic device for cell separation, cell lysis and DNA purification

Xing Chen et al.

ANALYTICA CHIMICA ACTA (2007)

Article Biochemical Research Methods

Cell lysis inside the capillary facilitated by transverse diffusion of laminar flow profiles (TDLFP)

Maxim V. Berezovski et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2007)

Article Infectious Diseases

Rapid tests for sexually transmitted infections (STIs): the way forward

R. W. Peeling et al.

SEXUALLY TRANSMITTED INFECTIONS (2006)

Article Chemistry, Physical

Preparation and morphology of SiO2/PMMA nanohybrids by microemulsion polymerization

Peng Xu et al.

COLLOID AND POLYMER SCIENCE (2006)

Article Biochemistry & Molecular Biology

Effects of phospholipid hydrolysis on the aggregate structure in DPPC/DSPE-PEG2000 liposome preparations after gel to liquid crystalline phase transition

Ludger M. Ickenstein et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2006)

Article Electrochemistry

Interfacial and electrokinetic characterization of IPA solutions related to semiconductor wafer drying and cleaning

Jin-Goo Park et al.

JOURNAL OF THE ELECTROCHEMICAL SOCIETY (2006)

Article Biochemistry & Molecular Biology

Effective interactions between chaotropic agents and proteins

G Salvi et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2005)

Article Biochemistry & Molecular Biology

Alcohol-mediated error-prone PCR

S Claveau et al.

DNA AND CELL BIOLOGY (2004)