Journal
TOXINS
Volume 8, Issue 12, Pages -Publisher
MDPI
DOI: 10.3390/toxins8120355
Keywords
Fusarium; mycotoxins; secondary metabolites; bioactivity; polyketides; pigments; antibiotics; bio-guided assays; antibacterial; aurofusarin
Categories
Funding
- Danish National Innovation Foundation [137-2012]
- Danish Agency for Science [4005-00204B, 0602-02612B]
Ask authors/readers for more resources
Bio-guided screening is an important method to identify bioactive compounds from fungi. In this study we applied a fast digital time-lapse microscopic method for assessment of the antibacterial properties of secondary metabolites from the fungal genus Fusarium. Here antibacterial effects could be detected for antibiotic Y, aurofusarin, beauvericin, enniatins and fusaric acid after six hours of cultivation. The system was then used in a bio-guided screen of extracts from 14 different Fusarium species, which had been fractionated by HPLC. In this screen, fractions containing the red pigments aurofusarin and bikaverin showed effects against strains of Lactobacillus and Bifidobacterium. The IC50 for aurofusarin against Lactobacillus acidophilus was 8 mu M, and against Bifidobacterium breve it was 64 mu M. Aurofusarin only showed an effect on probiotic bacteria, leading to the speculation that only health-promoting bacteria with a positive effect in the gut system are affected.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available