Rapid Information Retrieval from DNA Storage with Microfluidic Very Large-Scale Integration Platform

Due to its high information density, DNA is very attractive as a data storage system. However, a major obstacle is the high cost and long turnaround time for retrieving DNA data with next-generation sequencing. Herein, the use of a microfluidic very large-scale integration (mVLSI) platform is described to perform highly parallel and rapid readout of data stored in DNA. Additionally, it is demonstrated that multi-state data encoded in DNA can be deciphered with on-chip melt-curve analysis, thereby further increasing the data content that can be analyzed. The pairing of mVLSI network architecture with exquisitely specific DNA recognition gives rise to a scalable platform for rapid DNA data reading. A non-sequencing approach is demonstrated to perform high-throughput retrieval of information encoded within DNA. The concept makes use of the hybridization of fluorescent DNA probes to the information stored within DNA sequence to perform rapid readout in a microfluidic very large-scale integration (mVLSI) chip.image

Automated summary

DNA is an attractive data storage system due to its high information density. This study presents a microfluidic very large-scale integration (mVLSI) platform for highly parallel and rapid readout of DNA data. It also demonstrates the decoding of multi-state data encoded in DNA using on-chip melt-curve analysis, increasing the data content that can be analyzed.