Journal
STEM CELLS TRANSLATIONAL MEDICINE
Volume 5, Issue 5, Pages 658-669Publisher
WILEY
DOI: 10.5966/sctm.2015-0238
Keywords
Hypothermic storage; Human pluripotent stem cell-derived cardiomyocytes; Two-dimensional monolayer cultures; Three-dimensional aggregates; Cell therapy
Categories
Funding
- FP7 European Union Project Cardio Repair European Multidisciplinary Initiative [HEALTH-2009_242038]
- Fundacao para a Ciencia e Tecnologia (FCT)-funded project CARDIOSTEM [MITP-TB/ECE/0013/2013]
- Fundacao para a Ciencia e Tecnologia (FCT)-funded project CardioRegen [HMSP-ICT/0039/2013]
- Fundacao para a Ciencia e Tecnologia (FCT)-funded project CardioRecept [PTDC/BBB-B10/1414]
- iNOVA4Health [UID/Multi/04462/2013]
- FCT/Ministerio da Educacao e Ciencia, through national funds
- FEDER
- FCT [SFRH/BD/51573/2011]
- FCT Grant [SFRH/BD/51573/2011]
- Fundação para a Ciência e a Tecnologia [HMSP-ICT/0039/2013, MITP-TB/ECE/0013/2013, SFRH/BD/51573/2011] Funding Source: FCT
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To fully explore the potential of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs), efficient methods for storage and shipment of these cells are required. Here, we evaluated the feasibility to cold store monolayers and aggregates of functional CMs obtained from different PSC lines using a fully defined clinical-compatible preservation formulation and investigated the time frame that hPSC-CMs could be subjected to hypothermic storage. We showed that two-dimensional (2D) monolayers of hPSC-CMs can be efficiently stored at 4 degrees C for 3 days without compromising cell viability. However, cell viability decreased when the cold storage interval was extended to 7 days. We demonstrated that hPSC-CMs are more resistant to prolonged hypothermic storage-induced cell injury in three-dimensional aggregates than in 2D monolayers, showing high cell recoveries (>70%) after 7 days of storage. Importantly, hPSC-CMs maintained their typical (ultra)structure, gene and protein expression profile, electrophysiological profiles, and drug responsiveness.
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