4.8 Article

A Nano-Electroporation-DNA Tensioner Platform Enhances Intracellular Delivery and Mechanical Analysis Toward Rapid Drug Assessment

Related references

Note: Only part of the references are listed.
Article Nanoscience & Nanotechnology

All-Covalent Nuclease-Resistant and Hydrogel-Tethered DNA Hairpin Probes Map pN Cell Traction Forces

Sk Aysha Rashid et al.

Summary: Cells sense and respond to the physical properties of their environment through receptor-mediated signaling, a process known as mechanotransduction. Integrin forces transmitted by cells are highly sensitive to the substrate stiffness, with cells cultured on higher stiffness gels producing more hairpin unfolding events. This study provides a general strategy to integrate molecular tension probes into hydrogels and mimic in vivo mechanotransduction.

ACS APPLIED MATERIALS & INTERFACES (2023)

Article Biotechnology & Applied Microbiology

Electroactive nanoinjection platform for intracellular delivery and gene silencing

Ali-Reza Shokouhi et al.

Summary: A non-viral, low-voltage electroactive nanoinjection platform based on vertically configured conductive nanotubes (NTs) is proposed. The platform enables rapid delivery of targeted biomolecules into cells with minimal impact on cell viability, and allows for successful gene silencing. This new platform offers a safer and more versatile approach to intracellular delivery and has potential applications in cell engineering and gene silencing.

JOURNAL OF NANOBIOTECHNOLOGY (2023)

Article Chemistry, Multidisciplinary

Evaluating the therapeutic efficacy of nano-drugs targeting epidermal growth factor receptor

Jing Zhao et al.

Summary: The therapeutic efficacy of nano-drugs targeting EGFR was evaluated in terms of cell entry efficiency and induced cell mechanical properties at the single particle/cell level in real time.

CHEMICAL COMMUNICATIONS (2022)

Article Chemistry, Multidisciplinary

High-Throughput DNA Tensioner Platform for Interrogating Mechanical Heterogeneity of Single Living Cells

Xinxin Hang et al.

Summary: This study introduces a DNA tensioner platform with high throughput and pN-level resolution to detect the mechanical forces of individual cells. Using this platform, enhanced mechanical forces in drug-resistant cells and metastatic tumor cells, as well as the mechanical heterogeneity associated with genes VEGFA and MINK1, were identified. The platform has the potential to establish links between cellular mechanical heterogeneity and genetic heterogeneity.

SMALL (2022)

Article Biophysics

A finger-driven disposable micro-platform based on isothermal amplification for the application of multiplexed and point-of-care diagnosis of tuberculosis

Zhiying Wang et al.

Summary: A low-cost, portable finger-driven microfluidic chip was developed for rapid on-site detection of tuberculosis, showing unique advantages in avoiding risks of infection or environmental contamination and enabling multiplexed detection to differentiate different strains of Mycobacterium. In clinical trials, the platform demonstrated high specificity and sensitivity.

BIOSENSORS & BIOELECTRONICS (2022)

Article Biophysics

A dynamic and quantitative biosensing assessment for electroporated membrane evolution of cardiomyocytes

Mingyue Zhang et al.

Summary: The study develops a high-throughput, sensitive, and stable biosensing platform to dynamically and quantitatively assess the evolution of electroporated cell membrane. The platform explores and characterizes the processes of perforation, stabilization, and resealing of the cell membrane through intracellular recordings of action potentials.

BIOSENSORS & BIOELECTRONICS (2022)

Article Chemistry, Multidisciplinary

Sensitive Interrogation of Enhancer Activity in Living Cells on a Nanoelectroporation-Probing Platform

Fengqi Wan et al.

Summary: In this study, a nanoelectroporation-probing platform was developed for sensitive detection of enhancer activity in living cells. The probes targeting enhancer activity showed over 100-fold increase in fluorescence signals within 1 hour. The platform was used to detect the activity of CCAT1 enhancers in clinical lung cancer patients and revealed heterogeneity among different patients.

ACS SENSORS (2022)

Article Chemistry, Multidisciplinary

In Situ Inhibitor Synthesis and Screening by Fluorescence Polarization: An Efficient Approach for Accelerating Drug Discovery

Zhihong Li et al.

Summary: Target-directed dynamic combinatorial chemistry is an important tool in drug discovery, but faces challenges in analyzing complex mixtures. In this study, we propose a simple alternative called in situ inhibitor synthesis and screening (ISISS), which combines high-throughput bioorthogonal synthesis with screening for target binding using fluorescence. We demonstrate the effectiveness of the ISISS method in discovering a potent acylhydrazone-based inhibitor of human prolyl hydroxylase 2 (PHD2), a target for anemia treatment, with comparable in vivo potency to an approved medicine.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022)

Article Medicine, Research & Experimental

CRISPR/Cas9-mediated knockout of Lcn2 in human breast cancer cell line MDA-MB-231 ameliorates erastin-mediated ferroptosis and increases cisplatin vulnerability

Mehdi Rabiee Valashedi et al.

Summary: Targeting Lcn2 using CRISPR/Cas9 can inhibit cell proliferation and migration capability in breast cancer cells, enhance sensitivity to cisplatin, and promote ferroptotic cell death.

LIFE SCIENCES (2022)

Article Biochemical Research Methods

High-throughput and label-free enrichment of malignant tumor cells and clusters from pleural and peritoneal effusions using inertial microfluidics

Zhixian Zhu et al.

Summary: A polymer microfluidic chip was used for high-throughput and label-free enrichment of malignant tumor cells from pleural and peritoneal effusions. The enriched tumor cells were successfully purified and could be used for rapid drug sensitivity testing. This approach can accelerate cancer diagnosis and guide treatment medication selection.

LAB ON A CHIP (2022)

Article Nanoscience & Nanotechnology

Injectable and Self-Healing Hydrogels with Double-Dynamic Bond Tunable Mechanical, Gel-Sol Transition and Drug Delivery Properties for Promoting Periodontium Regeneration in Periodontitis

Huilong Guo et al.

Summary: Injectable hydrogels with self-healing properties and tunable sustained drug release through double-dynamic bonds can effectively promote periodontal regeneration in periodontitis. The hydrogels demonstrated excellent gelation process, injectability, and the ability to transition from gel to sol without external stimuli, making them promising candidates for controlled drug delivery in periodontal disease therapy.

ACS APPLIED MATERIALS & INTERFACES (2021)

Article Chemistry, Multidisciplinary

An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect

Yi-Fan Ruan et al.

Summary: A dual-functional photocathodic single-cell nanotool has been developed for direct intracellular drug delivery and evaluation of oxidative stress, pushing the boundary of current single-cell electroanalysis.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Article Biochemistry & Molecular Biology

Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro

Tina Batista Napotnik et al.

Summary: Electroporation is a successful method for intracellular drug delivery by temporarily permeabilizing plasma membrane with electric pulses. The orientation of cells in an electric field is crucial for electroporation and molecular transport. Different durations of electric pulses can impact the asymmetry of polar uptake of calcium ions, with longer pulses resulting in greater uptake at the cathode-side pole.

MOLECULES (2021)

Article Oncology

Knockout of vasohibin-2 reduces tubulin carboxypeptidase activity and increases paclitaxel sensitivity in ovarian cancer

Takahiro Koyanagi et al.

Summary: VASH2 knockout in ovarian cancer cells enhances chemosensitivity to paclitaxel by reducing tubulin detyrosination and upregulating cyclin B1 expression. This study suggests that targeting VASH2 may be a promising strategy for ovarian cancer treatment by inhibiting angiogenesis and modulating microtubule activity.

CANCER MEDICINE (2021)

Article Chemistry, Multidisciplinary

Single Living Cell Analysis Nanoplatform for High-Throughput Interrogation of Gene Mutation and Cellular Behavior

Zaizai Dong et al.

Summary: This study presents a novel single living cell analysis nanoplatform for interrogating gene properties and drug resistance simultaneously. By utilizing a specially designed Domino-probe and an on-chip addressable microwell-nanopore array, the system was validated in primary lung cancer cell samples, demonstrating a positive correlation between genes and cellular behaviors at the single-cell level.

NANO LETTERS (2021)

Article Multidisciplinary Sciences

Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules

Yuhong Cao et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Article Nanoscience & Nanotechnology

Intracellular Delivery and Sensing System Based on Electroplated Conductive Nanostraw Arrays

Rui Wen et al.

ACS APPLIED MATERIALS & INTERFACES (2019)

Article Chemistry, Multidisciplinary

Visualizing Intercellular Tensile Forces by DNA-Based Membrane Molecular Probes

Bin Zhao et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2017)

Article Chemistry, Multidisciplinary

Nanomechanically Visualizing Drug-Cell Interaction at the Early Stage of Chemotherapy

Yun-Long Wu et al.

ACS NANO (2017)

Article Nanoscience & Nanotechnology

Janus Silver-Mesoporous Silica Nanocarriers for SERS Traceable and pH-Sensitive Drug Delivery in Cancer Therapy

Dan Shao et al.

ACS APPLIED MATERIALS & INTERFACES (2016)

Article Biotechnology & Applied Microbiology

A Reductionist Metastasis-on-a-Chip Platform for In Vitro Tumor Progression Modeling and Drug Screening

Aleksander Skardal et al.

BIOTECHNOLOGY AND BIOENGINEERING (2016)

Article Chemistry, Multidisciplinary

Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging

Jing Gao et al.

NANOSCALE (2015)

Article Biotechnology & Applied Microbiology

Organs-on-chips at the frontiers of drug discovery

Eric W. Esch et al.

NATURE REVIEWS DRUG DISCOVERY (2015)

Article Nanoscience & Nanotechnology

Nanochannel electroporation delivers precise amounts of biomolecules into living cells

Pouyan E. Boukany et al.

NATURE NANOTECHNOLOGY (2011)

Article Cell Biology

The Kinetics of Force-Induced Cell Reorganization Depend on Microtubules and Actin

Alexandra M. Goldyn et al.

CYTOSKELETON (2010)

Article Biophysics

Monitoring cell adhesion by using thickness shear mode acoustic wave sensors

Fang Li et al.

BIOSENSORS & BIOELECTRONICS (2007)

Article Biochemical Research Methods

A single cell electroporation chip

M Khine et al.

LAB ON A CHIP (2005)