Journal
APPLIED CLAY SCIENCE
Volume 246, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.clay.2023.107196
Keywords
Nontronite; Glucose oxidase; Clay mineral nanozyme; Cascade platform; Glucose detection
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The combination of clay mineral nanozymes and natural enzymes has led to the construction of a high-performance enzymatic cascade system, which shows improved stability and catalytic efficiency. This work provides a simple and efficient reference for the design of enzyme systems and expands the application field of clay minerals.
Recently, the combination of nature enzymes and nanozymes provides new opportunities for constructing new generation of high-performance enzymatic cascade system. Herein, an enzyme cascade bioplatform (GOx-BSA/ NAu-2) has been constructed by combining clay mineral nanozymes (nontronite, NAu-2) and natural enzyme (glucose oxidase, GOx). NAu-2 can be used both as a carrier for GOx immobilization and as a mimic of peroxidase (POD) guiding an enzyme cascade bioplatform. Owing to the stabilizing effect of bovine serum albumin (BSA) on enzymes and proper immobilization method, the GOx-BSA/NAu-2 possess better thermal stability, acid-base stability, storage stability and reusability than free enzymes system. Given the substrate channel effect, the acquired GOx-BSA/NAu-2 exhibits higher catalytic efficiency than the BSA/NAu-2 system and the GOx + HRP system. This work provides an efficient and simple reference for the design of clay mineral nanozyme-enzyme bioplatform, and also broadens the application field of clay minerals.
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