Microscopic hyperspectral imaging and an improved detection model based detection of Mycogone perniciosa chlamydospore in soil

The Mycogone perniciosa disease of Agaricus bisporus is highly contagious, with insignificant early symptoms and a long infestation period. Currently, there is a lack of a convenient and rapid means to detect the disease for early control. By using the transmission route of the disease, this paper focuses on M. perniciosa chlamydospore detection in contaminated soil. Microscopic hyperspectral images of M. perniciosa chlamydospore in contaminated soil were obtained to establish a detection model. Given a small target and a complex background, we proposed an improved spore detection model based on a faster regional convolutional neural network (Faster RCNN). Furthermore, we combined the residual network Resnet50 and feature pyramid network (FPN) to extract thick spore target features at multiple scales. Meanwhile, we optimized the region proposal network (RPN) region proposal generation by adding two small scales to improve the performance of the detection model. The first three principal components (with 95% or more information) and RGB images were selected as model inputs, respectively, and the final average precision (AP) was 94.68% and 92.35%, respectively. This PC-based model also was compared to the VGG16 and Resnet50-based feature extraction networks of Faster R-CNN and Darknet53-based feature extraction network of the YOLOv3 model, and the AP was found to improve by 5.41%, 4.78%, and 6.34%, respectively. The results showed that micro-hyperspectral imaging combined with deep learning methods could accurately detect the chlamydospore in the soil, providing new methods and ideas for the early prevention and detection of M. perniciosa disease of A. bisporus.

Automated summary

This study successfully established a model for detecting the spores of Agaricus bisporus disease using hyperspectral imaging and deep learning methods, providing a new approach for early prevention and detection of the disease.