Solid-State Nanopore Sensors with Enhanced Sensitivity through Nucleic Acid Amplification

Solid-state nanopores have wide applications in DNA sequencing, energy conversion and storage, seawater desalination, sensors, and reactors due to their high stability, controllable geometry, and a variety of pore-forming materials. Solid-state nanopore sensors can be used for qualitative and quantitative analyses of ions, small molecules, proteins, and nucleic acids. The combination of nucleic acid amplification and solid-state nanopores to achieve trace detection of analytes is gradually attracting attention. This review outlines nucleic acid amplification strategies for enhancing the sensitivity of solid-state nanopore sensors by summarizing the articles published in the past 10 years. The future development prospects and challenges of nucleic acid amplification in solid-state nanopore sensors are discussed. This review helps readers better understand the field of solid-state nanopore sensors. We believe that solid-state nanopore sensors will break through the bottleneck of traditional detection and become a powerful single-molecule detection platform.

Automated summary

Solid-state nanopores have a wide range of applications in various fields, such as DNA sequencing, energy conversion and storage, seawater desalination, sensors, and reactors. They offer high stability and controllable geometry, making them suitable for qualitative and quantitative analysis of different molecules. This review summarizes the past decade's research on nucleic acid amplification strategies for enhancing the sensitivity of solid-state nanopore sensors and discusses the future prospects and challenges in this field.