Measuring macular pigment optical density using reflective images of confocal scanning laser system

Purpose To develop a method to measure the macular pigment optical density (MPOD) using scanning laser ophthalmoscopic images in young adults and children. Study design Cross-sectional study. Methods Blue light reflectance fundus images of 32 healthy subjects were used. A profile of the linear reflectance changes across the center of the fovea on a grayscale fundus image was generated. The ratio of the macula-to-periphery reflection was designated as the peak value of the MPOD (MPOD[FR]) based on established fundamentals. In the MPOD profile, the basal width of the pixels at MPOD < 0 (wMP) and width at one-half value of the MPOD[FR] (wMP(0.5)) were determined. The MOPD at eccentricity of 0.5 degrees was measured by heterochromatic flicker photometry (MPOD[HFP]), and the correlation between the MPOD[FR] and MPOD[HFP] was evaluated. Results The MPOD[FR] ranged from 0.17 to 0.73 with a mean of 0.40 +/- 0.13. The wMP ranged from 88 to 173 pixels with a mean of 121.7 +/- 24.2 pixels, and the wMP0.5 ranged from 38 to 83 pixels with a mean of 54.1 +/- 10.3 pixels. A significant correlation was found between the MPOD([FR] )and MPOD[HFP] (r = 0.41, P = 0.02). Conclusions This simplified method can provide accurate and reliable values of the MPOD comparable to heterochromatic flicker photometry. Obtaining the fundus images in this fast and easy way should be suitable for children thus enabling clinicians to determine the MPODs for children.

Automated summary

The purpose of this study was to develop a method to measure macular pigment optical density (MPOD) using scanning laser ophthalmoscopic images in young adults and children. The method involved generating a profile of linear reflectance changes across the center of the fovea on grayscale fundus images. The results showed that this simplified method provided accurate and reliable values of MPOD, comparable to heterochromatic flicker photometry. The method is fast and easy, making it suitable for use with children.