4.7 Article

Production of highly water-soluble genistein α-diglucoside using an engineered O-α-glycoligase with enhanced transglycosylation activity and altered substrate specificity

Related references

Note: Only part of the references are listed.
Article Agriculture, Multidisciplinary

Synthesis of Alpha-Linked Glucosides from Soybean Isoflavone Aglycones Using Amylosucrase from Deinococcus geothermalis

Young Sung Jung et al.

Summary: Using amylosucrase from Deinococcus geothermalis, alpha-linked glucosylation of Soybean isoflavone aglycones (SIAs) was achieved, resulting in 13 alpha-linked glucosyl SIAs with confirmed colors in solution. The structures of the compounds were identified using mass spectrometry and multidimensional nuclear magnetic resonance spectroscopy. The study suggests a strategy for the synthesis of bioactive compounds with enhanced water solubility through alpha-linked glucosylation, with the products attached to the C-4 ' of SIAs found to be more water-soluble than those attached to the C-7 and/or beta-linkages.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2023)

Article Biochemistry & Molecular Biology

pH-promoted O-α-glucosylation of flavonoids using an engineered α-glucosidase mutant

Chao Li et al.

Summary: An efficient O-alpha-glucosylation of flavonoids was developed using an O-alpha-glycoligase derived from a thermostable alpha-glucosidase, showing high yields and regioselectivity. The study provides insights into the acceptor specificity and regiospecificity of O-alpha-glucosylation by MalA-D416A, suggesting a potential general synthesis route for flavonoid O-alpha-glycosides.

BIOORGANIC CHEMISTRY (2021)

Article Multidisciplinary Sciences

Thioglycoligase derived from fungal GH3 β-xylosidase is a multi-glycoligase with broad acceptor tolerance

Manuel Nieto-Dominguez et al.

NATURE COMMUNICATIONS (2020)

Article Chemistry, Multidisciplinary

Diversion of a thioglycoligase for the synthesis of 1-O-acyl arabinofuranoses

Quentin Pavic et al.

CHEMICAL COMMUNICATIONS (2018)

Article Biochemistry & Molecular Biology

HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information

Lenka Sumbalova et al.

NUCLEIC ACIDS RESEARCH (2018)

Article Multidisciplinary Sciences

Engineering a branching sucrase for flavonoid glucoside diversification

Yannick Malbert et al.

SCIENTIFIC REPORTS (2018)

Article Biotechnology & Applied Microbiology

Eliminating hydrolytic activity without affecting the transglycosylation of a GH1 β-glucosidase

Pontus Lundemo et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2017)

Article Biotechnology & Applied Microbiology

High production of genistein diglucoside derivative using cyclodextrin glycosyltransferase from Paenibacillus macerans

Ruizhi Han et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2017)

Review Biochemistry & Molecular Biology

Advances in Enzymatic Glycoside Synthesis

Phillip M. Danby et al.

ACS CHEMICAL BIOLOGY (2016)

Article Biotechnology & Applied Microbiology

Improvement of the Optimum pH of Aspergillus niger Xylanase towards an Alkaline pH by Site-Directed Mutagenesis

Fei Li et al.

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY (2015)

Article Chemistry, Applied

Biphasic Catalysis with Disaccharide Phosphorylases: Chemoenzymatic Synthesis of α-D-Glucosides Using Sucrose Phosphorylase

Karel De Winter et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2014)

Article Biochemistry & Molecular Biology

Strategies for Modulating the pH-Dependent Activity of a Family 11 Glycoside Hydrolase

Martin L. Ludwiczek et al.

BIOCHEMISTRY (2013)

Article Biochemistry & Molecular Biology

α-Thioglycoligase-based synthesis of O-aryl α-glycosides as chromogenic substrates for α-glycosidases

Chao Li et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2013)

Article Biotechnology & Applied Microbiology

Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis

Chun You et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2012)

Article Chemistry, Multidisciplinary

Avogadro: an advanced semantic chemical editor, visualization, and analysis platform

Marcus D. Hanwell et al.

JOURNAL OF CHEMINFORMATICS (2012)

Article Biochemical Research Methods

Features and development of Coot

P. Emsley et al.

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (2010)

Article Thermodynamics

Solubility of Genistein in Water, Methanol, Ethanol, Propan-2-ol, 1-Butanol, and Ethyl Acetate from (280 to 333) K

Jian-Guo Wu et al.

JOURNAL OF CHEMICAL AND ENGINEERING DATA (2010)

Article Chemistry, Multidisciplinary

AutoDock4 and AutoDockTools4: Automated Docking with Selective Receptor Flexibility

Garrett M. Morris et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2009)

Article Agriculture, Multidisciplinary

Glycosylation of genistin into soluble inclusion complex form of cyclic glucans by enzymatic modification

D Li et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2005)

Article Biochemistry & Molecular Biology

Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts

J Mullegger et al.

PROTEIN ENGINEERING DESIGN & SELECTION (2005)

Article Biochemistry & Molecular Biology

Equations of substrate-inhibition kinetics applied to pig kidney diamine oxidase (DAO, EC 1.4.3.6)

G Ignesti

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY (2003)