Degradation of deoxynivalenol by a microbial consortia C1 from duck intestine

Deoxynivalenol (DON), one of the most widespread mycotoxins in food and feed, poses a persistent health threat to humans and farm animals, and is difficult to eliminate. The utilization of the biotransformation mechanism by microorganisms to detoxify DON is a promising strategy. Although individual strains are capable of DON degradation, their isolation and purification are challenging and time-consuming. Recently, the microbial consortia concept has been proposed, owing to their ability to perform more complex tasks and are more tolerant to environmental changes than individual strains or species. In this study, the novel microbial consortia C1 that could efficiently convert DON to de-epoxy DON (DOM-1) was screened from the cecum contents of ducks. After 24 h anaerobic incubation, 100 mu g/ml DON was completely degraded by C1. In vitro, C1 can effectively degrade DON in corn steep liquor (CSL) with an efficiency of 49.44% within 14 days. Furthermore, C1 effectively alleviated the DON poisoning in mice. After C1 treatment, the serum DON level decreased by 40.39%, and the reduction in serum total protein and albumin levels were mitigated. Additionally, C1 is effective in protecting the mouse liver against 5 mg/kg DON. These findings suggest that C1 could be a promising DON biological detoxifier and provide novel microbial resources for preventing DON contamination.

Automated summary

A microbial consortium called C1, which efficiently converts deoxynivalenol (DON) to de-epoxy DON (DOM-1), has been screened from the cecum contents of ducks. C1 can completely degrade 100 μg/ml DON after 24 h anaerobic incubation. In vitro, C1 can effectively degrade DON in corn steep liquor (CSL) and alleviate DON poisoning in mice. These findings suggest that C1 could be a promising biological detoxifier for DON and provide novel microbial resources for preventing DON contamination.