Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 23, Pages -Publisher
MDPI
DOI: 10.3390/ijms242316747
Keywords
dental stem cells; hDPSCs; osteogenesis; PLCL scaffold; HYAFF-11 scaffold
Ask authors/readers for more resources
This study investigates the osteogenic potential of human dental pulp stem cells (hDPSCs) grown on different scaffolds. Results show that a hydrolytically modified poly (L-lactide-co-caprolactone) (PLCL) electrospun scaffold provides a better environment for hDPSCs attachment and osteogenic differentiation compared to a non-woven hyaluronic acid (HYAFF-11 (TM)) mesh scaffold. The findings suggest that hDPSC/PLCL constructs could be a potential innovative approach for bone defect repair.
Bone tissue engineering using different scaffolds is a new therapeutic approach in regenerative medicine. This study explored the osteogenic potential of human dental pulp stem cells (hDPSCs) grown on a hydrolytically modified poly(L-lactide-co-caprolactone) (PLCL) electrospun scaffold and a non-woven hyaluronic acid (HYAFF-11 (TM)) mesh. The adhesion, immunophenotype, and osteogenic differentiation of hDPSCs seeded on PLCL and HYAFF-11 (TM) scaffolds were analyzed. The results showed that PLCL and HYAFF-11 (TM) scaffolds significantly supported hDPSCs adhesion; however, hDPSCs' adhesion rate was significantly higher on PLCL than on HYAFF-11 (TM). SEM analysis confirmed good adhesion of hDPSCs on both scaffolds before and after osteogenesis. Alizarin red S staining showed mineral deposits on both scaffolds after hDPSCs osteogenesis. The mRNA levels of runt-related transcription factor 2 (Runx2), collagen type I (Coll-I), osterix (Osx), osteocalcin (Ocn), osteopontin (Opn), bone sialoprotein (Bsp), and dentin sialophosphoprotein (Dspp) gene expression and their proteins were higher in hDPSCs after osteogenic differentiation on both scaffolds compared to undifferentiated hDPSCs on PLCL and HYAFF-11 (TM). These results showed that PLCL scaffolds provide a better environment that supports hDPSCs attachment and osteogenic differentiation than HYAFF-11 (TM). The high mRNA of early osteogenic gene expression and mineral deposits observed after hDPSCs osteogenesis on a PLCL mat indicated its better impact on hDPSCs' osteogenic potential than that of HYAFF-11 (TM), and hDPSC/PLCL constructs might be considered in the future as an innovative approach to bone defect repair.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available