☆ 4.8 Article

Isolation methods determine human neutrophil responses after stimulation

FRONTIERS IN IMMUNOLOGY (2023)

Related references

Note: Only part of the references are listed.

Review Immunology

Neutrophils in chronic inflammatory diseases

Andrea Herrero-Cervera et al.

Summary: Chronic inflammation is a common feature of many diseases and is especially prevalent in the aging population. The role of neutrophils in chronic inflammation is still not well understood, with conflicting evidence suggesting both beneficial and detrimental effects. However, emerging evidence indicates that neutrophils play a significant role in several chronic diseases.

CELLULAR & MOLECULAR IMMUNOLOGY (2022)

Add to Collection

Article Immunology

Specificity of mouse and human Fcgamma receptors and their polymorphic variants for IgG subclasses of different species

Yu Wang et al.

Summary: This study systematically assessed the binding specificities of IgG from different species to mouse and human Fcγ receptors. The results revealed specific binding patterns for each IgG subclass and identified potential issues with antibody-based immunoassays. This research provides a useful reference for the transition from animal models to preclinical mouse models or human cell-based bioassays.

EUROPEAN JOURNAL OF IMMUNOLOGY (2022)

Add to Collection

Article Multidisciplinary Sciences

Innate immune deficiencies are associated with severity and poor prognosis in patients with COVID-19

Marine Peyneau et al.

Summary: This study investigated the correlation between circulating innate phagocytes and disease severity in COVID-19 patients. The results showed abnormal phenotype of neutrophils and monocytes, elevated concentrations of activation markers in plasma. Critical patients exhibited increased immature and immunosuppressive neutrophils, downregulated HLA-DR monocytes, which were closely associated with disease severity. Additionally, impaired antimicrobial functions in neutrophils and monocytes of critical patients were correlated with organ dysfunction, severe infections, and mortality.

SCIENTIFIC REPORTS (2022)

Add to Collection

Article Immunology

Method Matters: Effect of Purification Technology on Neutrophil Phenotype and Function

Marfa Blanter et al.

Summary: This study compares the effects of classic density-gradient purification and immunomagnetic isolation on the phenotype and functionality of neutrophils. The results show that the two methods produce cells with different characteristics under different experimental conditions. Density-gradient-derived neutrophils exhibit increased polarization responses and release of reactive oxygen species, neutrophil extracellular traps, and granular proteins, while immunomagnetic isolation-derived neutrophils are mostly quiescent. The choice of purification method should depend on the specific experimental purposes.

FRONTIERS IN IMMUNOLOGY (2022)

Add to Collection

Article Multidisciplinary Sciences

Optimization of methods for the accurate characterization of whole blood neutrophils

Ashley N. Connelly et al.

Summary: This study investigates the effects of various methods for neutrophil characterization on neutrophil activation and degranulation levels. By comparing different methods, it is found that staining whole blood at low temperature and removing unbound antibodies prior to fixation and red blood cell lysis can minimize neutrophil activation, thus improving the consistency and reproducibility of neutrophil characterization.

SCIENTIFIC REPORTS (2022)

Add to Collection

Article Biochemical Research Methods

Determination of reference intervals for neutrophil granular enzymes is affected by cell isolation techniques br

Devora London et al.

Summary: The study found that the activity of NE and MPO depended on the neutrophil isolation technique, with immunomagnetic negative selection showing favorable outcomes in terms of NE and MPO activity research. Care must be taken when interpreting NE and MPO activity data in clinical practice, depending on the cell isolation technique used.

JOURNAL OF IMMUNOLOGICAL METHODS (2022)

Add to Collection

Article Cell Biology

Reducing neutrophil exposure to oxygen allows their basal state maintenance

Louise Injarabian et al.

Summary: Neutrophils are highly glycolytic cells that are susceptible to oxygen exposure, resulting in their activation and death. Culturing neutrophils in hyperoxic conditions leads to hyper-activation and spontaneous release of neutrophil extracellular traps, impacting cytosolic protein composition. Manipulating neutrophils under hyperoxic conditions during purification can alter protein composition and should be avoided to maintain neutrophil function.

IMMUNOLOGY AND CELL BIOLOGY (2021)

Add to Collection

Article Immunology

Activation of Neutrophil Granulocytes by Platelet-Activating Factor Is Impaired During Experimental Sepsis

Stefan Hug et al.

Summary: Platelet-activating factor (PAF) plays a crucial role in the systemic inflammatory response, particularly in sepsis. This study investigates the changes in neutrophil cellular responses induced by PAF during sepsis, focusing on membrane potential, intracellular pH, and cellular swelling. The study found that in a pathological environment, the response of neutrophils to PAF was altered, with exposure to lipopolysaccharide leading to diminished intracellular alkalization and neutrophil size changes. These findings suggest that an impaired physiological response of neutrophils to PAF stimulation may contribute to the development of neutrophil dysfunction in sepsis.

FRONTIERS IN IMMUNOLOGY (2021)

Add to Collection

Review Immunology

The Neutrophil

Garth Lawrence Burn et al.

Summary: Neutrophils are immune cells with potent antimicrobial properties, capable of efficiently killing microorganisms both intracellularly and extracellularly. In addition to fighting infections, they can use their toxic machinery in other contexts such as cancer. However, inappropriate or dysregulated activation of neutrophils can lead to host damage and contribute to autoimmune and inflammatory diseases.

IMMUNITY (2021)

Add to Collection

Article Multidisciplinary Sciences

TICAM2-related pathway mediates neutrophil exhaustion

RuiCi Lin et al.

SCIENTIFIC REPORTS (2020)

Add to Collection

Article Multidisciplinary Sciences

Blood collection technique, anticoagulants and storing temperature have minor effects on the isolation of polymorphonuclear neutrophils

Julia Krabbe et al.

SCIENTIFIC REPORTS (2020)

Add to Collection

Article Cell Biology

An IgG-induced neutrophil activation pathway contributes to human drug-induced anaphylaxis

Friederike Jonsson et al.

SCIENCE TRANSLATIONAL MEDICINE (2019)

Add to Collection

Article Immunology

Neutrophil extracellular traps drive inflammatory pathogenesis in malaria

Sebastian Lorenz Knackstedt et al.

SCIENCE IMMUNOLOGY (2019)

Add to Collection

Article Hematology

A comparison of different methods of red blood cell leukoreduction and additive solutions on the accumulation of neutrophil-priming activity during storage

Michele M. Loi et al.

TRANSFUSION (2018)

Add to Collection

Article Multidisciplinary Sciences

Chromatin swelling drives neutrophil extracellular trap release

Elsa Neubert et al.

NATURE COMMUNICATIONS (2018)

Add to Collection

Review Immunology

Neutrophils: New insights and open questions

Klaus Ley et al.

SCIENCE IMMUNOLOGY (2018)

Add to Collection

Review Immunology

Neutrophil extracellular traps in immunity and disease

Venizelos Papayannopoulos

NATURE REVIEWS IMMUNOLOGY (2018)

Add to Collection

Article Immunology

Platelets expressing IgG receptor Fc gamma RIIA/CD32A determine the severity of experimental anaphylaxis

Heloise Beutier et al.

SCIENCE IMMUNOLOGY (2018)

Add to Collection

Letter Allergy

The importance of being pure neutrophils

Federica Calzetti et al.

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY (2017)

Add to Collection

Article Cell Biology

Human blood monocytes are able to form extracellular traps

Vanessa Granger et al.

JOURNAL OF LEUKOCYTE BIOLOGY (2017)

Add to Collection

Review Multidisciplinary Sciences

In vitro induction of NETosis: Comprehensive live imaging comparison and systematic review

Tamara Hoppenbrouwers et al.

PLOS ONE (2017)

Add to Collection

Article Biology

Diverse stimuli engage different neutrophil extracellular trap pathways

Elaine F. Kenny et al.

ELIFE (2017)

Add to Collection

Article Medicine, General & Internal

Severe congenital neutropenias

Julia Skokowa et al.

NATURE REVIEWS DISEASE PRIMERS (2017)

Add to Collection

Review Immunology

Current Understanding in Neutrophil Differentiation and Heterogeneity

Chang-Won Hong

IMMUNE NETWORK (2017)

Add to Collection

Review Immunology

Neutrophils as protagonists and targets in chronic inflammation

Oliver Soehnlein et al.

NATURE REVIEWS IMMUNOLOGY (2017)

Add to Collection

Article Medicine, Research & Experimental

Antimicrobial activity of HL-60 cells compared to primary blood-derived neutrophils against Staphylococcus aureus

Ragheda Yaseen et al.

JOURNAL OF NEGATIVE RESULTS IN BIOMEDICINE (2017)

Add to Collection

Article Hematology

Human neutrophil kinetics: modeling of stable isotope labeling data supports short blood neutrophil half-lives

Julio Lahoz-Beneytez et al.

BLOOD (2016)

Add to Collection

Review Cell Biology

Red cell DAMPs and inflammation

Rafaela Mendonca et al.

INFLAMMATION RESEARCH (2016)

Add to Collection

Article Dermatology

Dimethylfumarate Impairs Neutrophil Functions

Susen Mueller et al.

JOURNAL OF INVESTIGATIVE DERMATOLOGY (2016)

Add to Collection

Article Education, Scientific Disciplines

Primary immune deficiencies with defects in neutrophil function

Mary C. Dinauer

HEMATOLOGY-AMERICAN SOCIETY OF HEMATOLOGY EDUCATION PROGRAM (2016)

Add to Collection

Article Education, Scientific Disciplines

Autoimmune and other acquired neutropenias

Peter E. Newburger

HEMATOLOGY-AMERICAN SOCIETY OF HEMATOLOGY EDUCATION PROGRAM (2016)

Add to Collection

Article Multidisciplinary Sciences

Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus

Han Wei Hou et al.

SCIENTIFIC REPORTS (2016)

Add to Collection

Article Hematology

Flow cytometric assay for direct quantification of neutrophil extracellular traps in blood samples

Mathilde Gavillet et al.

AMERICAN JOURNAL OF HEMATOLOGY (2015)

Add to Collection

Article Multidisciplinary Sciences

Whose Gene Is It Anyway? The Effect of Preparation Purity on Neutrophil Transcriptome Studies

Huw B. Thomas et al.

PLOS ONE (2015)

Add to Collection

Article Immunology

Immobilized Immune Complexes Induce Neutrophil Extracellular Trap Release by Human Neutrophil Granulocytes via FcγRIIIB and Mac-1

Martina Behnen et al.

JOURNAL OF IMMUNOLOGY (2014)

Add to Collection

Article Cell Biology

Technical Advance: Autofluorescence-based sorting: rapid and nonperturbing isolation of ultrapure neutrophils to determine cytokine production

David A. Dorward et al.

JOURNAL OF LEUKOCYTE BIOLOGY (2013)

Add to Collection

Article Multidisciplinary Sciences

Cytokines Induced Neutrophil Extracellular Traps Formation: Implication for the Inflammatory Disease Condition

Ravi S. Keshari et al.

PLOS ONE (2012)

Add to Collection

Article Immunology

Human neutrophils switch to an activated phenotype after homing to the lung irrespective of inflammatory disease

E. Fortunati et al.

CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2009)

Add to Collection

Article Critical Care Medicine

Alteration of polymorphonuclear neutrophil surface receptor expression and migratory activity after isolation: Comparison of whole blood and isolated PMN preparations from normal and postfracture trauma patients

I Pallister et al.

JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE (2006)

Add to Collection

Article Biochemistry & Molecular Biology

CD15 expression in mature granulocytes is determined by α1,3-fucosyltransferase IX,but in promyelocytes and monocytes by α1,3-fucosyltransferase IV

F Nakayama et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2001)

Add to Collection

© Peeref 2019-2024. All rights reserved.