Journal
FEMS MICROBIOLOGY LETTERS
Volume 370, Issue -, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/femsle/fnad125
Keywords
metabolomics; multi-omics; biofilm; fish microbiome; lake microbiome; cyanobacteria
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Massive sequencing of the 16S rRNA gene is a standard method for describing and comparing microbial communities. Nontargeted metabolomics can complement this by providing information on community functioning. This study validates a method of extracting metabolites followed by DNA extraction, which allows for the combination of 16S rRNA sequencing with metabolome characterization from a single sample.
Massive sequencing of the 16S rRNA gene has become a standard first step to describe and compare microbial communities from various samples. Parallel analysis of high numbers of samples makes it relevant to the statistical testing of the influence of natural or experimental factors and variables. However, these descriptions fail to document changes in community or ecosystem functioning. Nontargeted metabolomics are a suitable tool to bridge this gap, yet extraction protocols are different. In this study, prokaryotic community compositions are documented by 16S rRNA gene sequencing after direct DNA extraction or after metabolites extraction followed by DNA extraction. Results obtained using the V3-V4 region on nonaxenic cultures of cyanobacteria, lake water column, biofilm, and gut of wild and lab-reared fish indicate that prior extraction of metabolites does not influence the obtained image of prokaryotic communities. This validates sequential extraction of metabolites followed by DNA as a way to combine 16S rRNA sequencing with metabolome characterization from a single sample. This approach has the potential to complement community structure characterization with a proxy of their functioning, without the uncertainties associated with the use of separate samples. Prior metabolite extraction does not alter the 16S rRNA-based characterization of the prokaryotic community.
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