Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 24, Issue 23, Pages -Publisher
MDPI
DOI: 10.3390/ijms242317109
Keywords
extracellular vesicle; umbilical-cord-derived mesenchymal stem cells; SIRT1; p53; NF-kappa B
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The protective effects of extracellular vesicles derived from human umbilical cord mesenchymal stem cells (UCMSC-EVs) against oxazolone-induced damage in HaCaT cells were investigated. UCMSC-EVs pretreatment elevated cell viability, reduced intracellular ROS, and prevented changes in mitochondrial membrane potential. Furthermore, UCMSC-EVs exhibited anti-inflammatory activity by reducing the expression of inflammatory cytokines.
The protective roles of extracellular vesicles derived from human umbilical cord mesenchymal stem cells against oxazolone-induced damage in the immortalized human keratinocyte cell line HaCaT were investigated. The cells were pretreated with or without UCMSC-derived extracellular vesicles 24 h before oxazolone exposure. The pretreated UVMSC-EVs showed protective activity, elevating cell viability, reducing intracellular ROS, and reducing the changes in the mitochondrial membrane potential compared to the cells with a direct oxazolone treatment alone. The UCMSC-EVs exhibited anti-inflammatory activity via reducing the inflammatory cytokines IL-1 beta and TNF-alpha. A mechanism study showed that the UCMSC-EVs increased the protein expression levels of SIRT1 and P53 and reduced P65 protein expression. It was concluded that UVMSC-EVs can induce the antioxidant defense systems of HaCaT cells and that they may have potential as functional ingredients in anti-aging cosmetics for skin care.
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