Journal
POLYMERS
Volume 15, Issue 22, Pages -Publisher
MDPI
DOI: 10.3390/polym15224420
Keywords
freeze drying; cardiac tissue engineering; polymer processing; anisotropic porosity
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This article investigates the reconstruction of the helical arrangement of cardiac muscle fibers using ice templating technology to develop functional artificial tissue. The study demonstrates the control of pore alignment direction through base topography, with an observed gradual shift of 106 ± 10 degrees, providing the flexibility to adjust pore size and spatial helical angle distribution for personalized medicine.
The helical arrangement of cardiac muscle fibres underpins the contractile properties of the heart chamber. Across the heart wall, the helical angle of the aligned fibres changes gradually across the range of 90-180 degrees. It is essential to recreate this structural hierarchy in vitro for developing functional artificial tissue. Ice templating can achieve single-oriented pore alignment via unidirectional ice solidification with a flat base mould design. We hypothesise that the orientation of aligned pores can be controlled simply via base topography, and we propose a scalable base design to recapitulate the transmural fibre orientation. We have utilised finite element simulations for rapid testing of base designs, followed by experimental confirmation of the Bouligand-like orientation. X-ray microtomography of experimental samples showed a gradual shift of 106 +/- 10 degrees, with the flexibility to tailor pore size and spatial helical angle distribution for personalised medicine.
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