Microstructural and histochemical analysis of shoots and cones of Juniperus seravschanica (Cupressaceae)

Juniper species contain abundant compounds that are used in the medicine, cosmetic, and wood industry. Furthermore, these components protect the genus against herbivores, pathogens and detrimental abiotic conditions. Stains and specific reagents can be used individually or simultaneously to mark cell shape, arrangement and the material they are made from. Microchemical analyses using specific reagents and stains under light microscopy are helpful for the characterization of chemical compounds present in plant tissues. The autofluorescence of endogenous fluorophores is used to enable their localization in plant cells and tissues. This paper aims to investigate the cytochemical and histochemical traits of the shoots (leaves and stems) and female cones (berries) of Juniperus seravschanica. Light and florescent microscopy techniques were used to analyze the cytology and localization of different compounds for the first time. Microscopy-based histochemical analyses revealed various products in terms of composition and distribution among the shoots and female cones. These specific compounds contained lignin, tannins, polysaccharides, starch, phenolic compounds, chlorophyll, terpenoids, neutral lipids, and proteins. However, the anatomical position of each metabolite and its concentration was different among leaf, stem, and female cone. Phenolic cells of young cones were differentiated into sclereid cells during development. The density of phenolic cells, sclereid cells, and resin glans was higher in female cones than leaves and stems. The high levels of various components can be related to high resistance of the species against biotic and abiotic stresses, confirm its industrial, pharmaceutical and agricultural applications and is useful for identification of diagnostic taxonomic traits. Research Highlights center dot Microscopical and histochemical analyses showed various compounds in J. seravschanica center dot The phenolic cells differentiated to sclereid cells during development center dot High levels of idioblasts and various compounds show its high resistance and medicinal role

Automated summary

Juniper species contain abundant compounds that have various applications and protect the plants from stress. This study investigated the cytochemical and histochemical traits of Juniperus seravschanica, revealing the composition and distribution of different compounds in leaves, stems, and female cones. The high levels of various compounds in the female cones contribute to their resistance and medicinal role.