4.6 Article

Genome of Linum usitatissimum convar. crepitans expands the view on the section Linum

Journal

FRONTIERS IN GENETICS
Volume 14, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fgene.2023.1269837

Keywords

flax; Linum usitatissimum convar. crepitans; section Linum; nanopore sequencing; de novo genome assembly

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Sequencing the genome of flax provides valuable genetic information for studying its evolution, domestication, and genome regulation. In this study, we obtained a high-quality genome assembly of Linum usitatissimum convar. crepitans using the Canu-Racon x2-medaka-POLCA approach.
Sequencing whole plant genomes provides a solid foundation for applied and basic studies. Genome sequences of agricultural plants attract special attention, as they reveal information on the regulation of beneficial plant traits. Flax is a valuable crop cultivated for oil and fiber. Genome sequences of its representatives are rich sources of genetic information for the improvement of cultivated forms of the plant. In our work, we sequenced the first genome of flax with the dehiscence of capsules-Linum usitatissimum convar. crepitans (Boenn.) Dumort-on the Oxford Nanopore Technologies (ONT) and Illumina platforms. We obtained 23 Gb of raw ONT data and 89 M of 150 + 150 paired-end Illumina reads and tested different tools for genome assembly and polishing. The genome assembly produced according to the Canu-Racon x2-medaka-POLCA scheme had optimal contiguity and completeness: assembly length-412.6 Mb, N50-5.2 Mb, L50-28, and complete BUSCO-94.6% (64.0% duplicated, eudicots_odb10). The obtained high-quality genome assembly of L. usitatissimum convar. crepitans provides opportunities for further studies of evolution, domestication, and genome regulation in the section Linum.

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