Gastrodia elata specific miRNA attenuates neuroinflammation via modulating NF-κB signaling pathway

Aims: Based on our previous research on the specific miRNAs identified from Gastrodia elata, we selected Gas-miR2-3p to investigate its effects on neuroinflammation via in vitro and in vivo experiments.Results: RT-qPCR analysis indicated that G. elata specific Gas-miR2-3p was detected in all murine tissues post-oral administration, suggesting their potential as orally bioavailable miRNA. The analysis of RT-qPCR, Western blotting and ELISA assays consistently demonstrate that the expression of inflammatory factors as TNF-alpha, IL-6, IL-1 beta was decreased and the expression levels of p-p65 and p-I kappa B alpha were downregulated after the action of Gas-miR2-3p in both cell and animal experiments.Conclusion: Gas-miR2-3p can attenuate neuroinflammation by regulating the inflammation factors and suppressing the activation of the NF-kappa B signaling pathway. Our findings indicate that G. elata miRNAs, as novel active components, perform a modulatory role in the NF-kappa B signaling pathway associated with neuroinflammation in a cross-species way.

Automated summary

Based on the experiments, it was found that Gas-miR2-3p from Gastrodia elata can attenuate neuroinflammation by regulating inflammatory factors and suppressing the activation of the NF-kappa B signaling pathway. This suggests that G. elata miRNAs have a modulatory role in neuroinflammation across different species.