Journal
HUMAN CELL
Volume -, Issue -, Pages -Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s13577-023-01008-z
Keywords
Amniotic fluid; Heterogeneity; Mesenchymal stem cells; Sepsis
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Amniotic fluid derived mesenchymal stem cells (AFMSCs) exhibit superior multipotency and immunomodulatory properties compared to MSCs derived from other somatic tissues. This study categorized AFMSCs into three distinct groups and found that a minority population (AFMSCs-X) displayed the highest multipotency and immunomodulatory abilities. Experimental results in a septic mice model showed that AFMSCs-X significantly improved survival rates and reduced bacterial colonization.
Amniotic fluid derived mesenchymal stem cells (AFMSCs), shed along the fetal development, exhibit superior multipotency and immunomodulatory properties compared to MSCs derived from other somatic tissues (e.g., bone marrow and fat). However, AFMSCs display heterogeneity due to source ambiguity, making them an underutilized stem cells source for translational clinical trials. Consequently, there is an urgent need to identify a method to purify the AFMSCs for clinical use. We found that the AFMSCs can be categorized into three distinct groups: kidney-specific AFMSCs (AFMSCs-K), lung-specific AFMSCs (AFMSCs-L), and AFMSCs with an undefined tissue source (AFMSCs-X). This classification was based on tissue-specific gene expression pattern of single cell colony. Additionally, we observed that AFMSCs-X, a minority population within the AFMSCs, exhibited the highest multipotency, proliferation, resistance to senescence and immuno-modulation. Our results showed that AFMSCs-X significantly improved survival rates and reduced bacterial colony forming units (CFU) in cecal ligation and puncture (CLP)-induced septic mice. Therefore, our study introduces a novel classification method to enhance the consistency and efficacy of AFMSCs. These subpopulations, originating from different tissue source, may offer a valuable and innovative resource of cells for regenerative medicine purposes.
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