A review on methods for extracting and quantifying microplastic in biological tissues

Literature about the occurrence of microplastic in biological tissues has increased over the last few years. This review aims to synthesis the evidence on the preparation of biological tissues, chemical identification of microplastic and accumulation in tissues. Several microplastic's extraction approaches from biological tissues emerged (i.e., alkaline, acids, oxidizing and enzymatic). However, criteria used for the selection of the extraction method have yet to be clarified. Similarly, analytical methodologies for chemical identification often does not align with the size of particles. Furthermore, sizes of microplastics found in biological tissues are likely to be biologically implausible, due to the size of the biological barriers. From this review, it emerged that further assessment are required to determine whether microplastic particles were truly internalized, were in the vasculature serving these organs, or were an artefact of the methodological process. The importance of a standardisation of quality control/quality assurance emerged. Findings arose from this review could have a broad implication, and could be used as a basis for further investigations, to reduce artifact results and clearly assess the fate of microplastics in biological tissues.

Automated summary

The literature on microplastics in biological tissues has increased in recent years. This review synthesizes evidence on the preparation of biological tissues, chemical identification of microplastics, and their accumulation in tissues. Issues such as unclear criteria for selecting extraction methods and inconsistent analytical methodologies for chemical identification have been identified. The sizes of microplastics found in biological tissues may be biologically implausible, and further assessment is needed to determine their true presence.