☆ 4.6 Article

Electrokinetic focusing of SARS-CoV-2 spike protein via ion concentration polarization in a paper-based lateral flow assay

ANALYTICAL METHODS (2023)

Related references

Note: Only part of the references are listed.

Article Biochemical Research Methods

Low-Powered pH-Stable Nano-electrokinetically Enhanced Lateral Flow Assay for COVID-19 Antigen Test

Kang Hyeon Kim et al.

Summary: Lateral flow assay (LFA) is a popular diagnostic system in point-of-care testing (POCT) due to its low cost and portability, but it has limited sensitivity and detection limits. Preconcentration through nano-electrokinetic (NEK) techniques has been proposed as a promising solution to improve the sensitivity of LFA. To address these limitations, an integrated LFA kit, the NEK-enhanced LFA (PcNEK-LFA), has been introduced. This kit features a pH-controlled structure designed to facilitate sample preconcentration, resulting in enhanced performance.

BIOCHIP JOURNAL (2023)

Add to Collection

Review Chemistry, Analytical

Recent advances in microscale extraction driven by ion concentration polarization

Aparna Krishnamurthy et al.

Summary: Microscale extraction offers advantages in sample throughput, specificity, and efficiency, which can be utilized to improve assays, sensor signals, and sample purification. Electrokinetic microextraction techniques have gained attention for their control over mass transport, ease of actuation, and portability, making them suitable for microscale and point-of-care devices. While most articles focus on analyte preconcentration using ion concentration polarization (ICP), few have explored the subsequent extraction step. This review discusses recent advancements in ICP-mediated microfluidic extraction methods and their potential for further development.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2022)

Add to Collection

Article Virology

Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification

Nicky Craig et al.

Summary: Studying the complete virus replication cycle of SARS-CoV-2 is crucial for identifying host factors and treatments. A direct lysis RT-qPCR method was developed that is fast, accurate, sensitive, and cost-effective for quantifying SARS-CoV-2 in culture supernatant. This method has wider applicability to other enveloped viruses.

VIRUSES-BASEL (2022)

Add to Collection

Article Biophysics

Nanoelectrokinetic-assisted lateral flow assay for COVID-19 antibody test

Cheonjung Kim et al.

Summary: A nanoelectrokinetic-assisted lateral flow assay platform is proposed to enhance the sensitivity and limit of detection for self-testing, especially in the context of the COVID-19 pandemic, by using a microfluidic paper-based preconcentrator. This preconcentration technique improved the performance of the lateral flow assay by 32-fold and increased the analytical sensitivity by 16.4%, offering a new opportunity for point-of-care testing and self-testing.

BIOSENSORS & BIOELECTRONICS (2022)

Add to Collection

Review Chemistry, Analytical

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

Wesley Wei-Wen Hsiao et al.

Summary: This review discusses the importance of developing reliable and robust diagnostic tests to limit the spread of COVID-19, highlighting the advantages and limitations of lateral flow technologies, as well as exploring the integration of smartphones and Artificial Intelligence for disease control and surveillance in the future.

BIOSENSORS-BASEL (2021)

Add to Collection

Article Biophysics

A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

Jong-Hwan Lee et al.

Summary: This study presents a novel rapid detection method for the SARS-CoV-2 S1 antigen using a matched pair of ACE2 and antibody. The detection method showed high specificity and sensitivity without cross-reactivity, making it a valuable tool in detecting the S1 antigen from COVID-19 patients.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Review Chemistry, Analytical

Enhancing the analytical performance of paper lateral fl ow assays: From chemistry to engineering

Vahid Shirshahi et al.

Summary: Paper-based lateral flow assays (LFAs) have achieved significant success in point-of-care diagnostics, however, they still face challenges in sensitivity, specificity, selectivity, reproducibility, and multiplex capability. Potential solutions in chemistry, nanobiotechnology, and engineering can be applied to enhance sensitivity, improve specificity, optimize selectivity, and develop better controls for reproducibility, ultimately paving the way for more efficient LFAs in various fields such as clinical settings, food safety, environment monitoring, and biosecurity.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis

Yilin Liu et al.

Summary: LFAs, paper-based point-of-care diagnostic tools, have traditionally lower sensitivities and specificities than standard laboratory tests. Recent efforts have focused on optimizing assay kinetics, signal amplification, and sample preamplification to increase sensitivities, as well as improving specificity through high-affinity molecules and assay optimization. With continuing improvements, LFAs may soon offer performance competitive with laboratory techniques while retaining a rapid format.

ACS NANO (2021)

Add to Collection

Article Medical Laboratory Technology

Performance evaluation of a lateral flow assays for nasopharyngeal antigen detection for SARS-CoV-2 diagnosis

Marcela Pena-Rodrigez et al.

Summary: This study evaluated the performance of a chromatographic immunoassay for the rapid diagnosis of SARS-CoV-2 antigen in Western Mexico through a cross-sectional study. It found that the rapid antigen test showed high sensitivity and specificity for SARS-CoV-2 antigen, with symptoms like anosmia being significant factors for positive diagnosis in both tests.

JOURNAL OF CLINICAL LABORATORY ANALYSIS (2021)

Add to Collection

Article Infectious Diseases

Additional Positive Electric Residues in the Crucial Spike Glycoprotein S Regions of the New SARS-CoV-2 Variants

Piotr H. Pawlowski

Summary: This study analyzed the changes in formal charge of 34 SARS-CoV-2 lineages from September 2020 to June 2021 and found that positive mutations, especially in the spike protein, were associated with an increase in COVID-19 case fatality rate. The data mining confirmed the importance of additional positive charge in enabling virus impact on immunity.

INFECTION AND DRUG RESISTANCE (2021)

Add to Collection

Review Biochemistry & Molecular Biology

Protein-based lateral flow assays for COVID-19 detection

Farbod Mahmoudinobar et al.

Summary: In order to combat the spread of COVID-19, rapid diagnostic innovations based on protein-protein interactions have been developed, particularly antigen tests using the LFA test platform. Despite the convenience and lower cost of these tests, they have faced criticism for lower sensitivity, prompting recent efforts to create rapid LFA tests with higher limit of detection.

PROTEIN ENGINEERING DESIGN & SELECTION (2021)

Add to Collection

Article Biochemical Research Methods

Rapid pre-concentration of Escherichia coli in a microfluidic paper-based device using ion concentration polarization

A. T. K. Perera et al.

ELECTROPHORESIS (2020)

Add to Collection

Article Biochemical Research Methods

Paper-based nuclease protection assay with on-chip sample pretreatment for point-of-need nucleic acid detection

Eka Noviana et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2020)

Add to Collection

Review Biochemical Research Methods

Physicochemical properties of SARS-CoV-2 for drug targeting, virus inactivation and attenuation, vaccine formulation and quality control

Christin Scheller et al.

ELECTROPHORESIS (2020)

Add to Collection

Article Immunology

Sensitivity in Detection of Antibodies to Nucleocapsid and Spike Proteins of Severe Acute Respiratory Syndrome Coronavirus 2 in Patients With Coronavirus Disease 2019

Peter D. Burbelo et al.

JOURNAL OF INFECTIOUS DISEASES (2020)

Add to Collection

Review Chemistry, Analytical

Tutorial review: Enrichment and separation of neutral and charged species by ion concentration polarization focusing

Beatrise Berzina et al.

ANALYTICA CHIMICA ACTA (2020)

Add to Collection

Article Biochemical Research Methods

Analysis of pressure-driven membrane preconcentration for point-of-care assays

A. Drexelius et al.

BIOMICROFLUIDICS (2020)

Add to Collection

Letter Pathology

The impact of viral transport media on PCR assay results for the detection of nucleic acid from SARS-CoV-2

P. D. Kirkland et al.

PATHOLOGY (2020)

Add to Collection

Article Chemistry, Multidisciplinary

Beyond Shielding: The Roles of Glycans in the SARS-CoV-2 Spike Protein

Lorenzo Casalino et al.

ACS CENTRAL SCIENCE (2020)

Add to Collection

Article Nanoscience & Nanotechnology

Detection of urine protein by a paper-based analytical device enhanced with ion concentration polarization effect

Han Gao et al.

MICROFLUIDICS AND NANOFLUIDICS (2019)

Add to Collection

Article Chemistry, Analytical

Magnetic field assisted preconcentration of biomolecules for lateral flow assaying

Antareep Sharma et al.

SENSORS AND ACTUATORS B-CHEMICAL (2019)

Add to Collection

Review Biochemical Research Methods

Sensitivity enhancement in lateral flow assays: a systems perspective

Joshua D. Bishop et al.

LAB ON A CHIP (2019)

Add to Collection

Article Chemistry, Analytical

An Electrokinetic Separation Route to Source Dialysate from Excess Fluid in Blood

Beatrise Berzina et al.

ANALYTICAL CHEMISTRY (2018)

Add to Collection

Article Biochemical Research Methods

Battery operated preconcentration-assisted lateral flow assay

Cheonjung Kim et al.

LAB ON A CHIP (2017)

Add to Collection

Article Chemistry, Analytical

Faradaic Ion Concentration Polarization on a Paper Fluidic Platform

Xiang Li et al.

ANALYTICAL CHEMISTRY (2017)

Add to Collection

Article Chemistry, Analytical

Spatiotemporally Defining Biomolecule Preconcentration by Merging Ion Concentration Polarization

Rhokyun Kwak et al.

ANALYTICAL CHEMISTRY (2016)

Add to Collection

Article Biochemical Research Methods

Microfluidic paper-based biomolecule preconcentrator based on ion concentration polarization

Sung Il Han et al.

LAB ON A CHIP (2016)

Add to Collection

Article Biochemical Research Methods

Sample pre-concentration with high enrichment factors at a fixed location in paper-based microfluidic devices

Shih-Hao Yeh et al.

LAB ON A CHIP (2016)

Add to Collection

Article Chemistry, Analytical

High throughput and multiplex localization of proteins and cells for in situ micropatterning using pneumatic microfluidics

Jian-Chun Wang et al.

ANALYST (2015)

Add to Collection

Article Chemistry, Analytical

Two Orders of Magnitude Improvement in Detection Limit of Lateral Flow Assays Using lsotachophoresis

Babak Y. Moghadam et al.

ANALYTICAL CHEMISTRY (2015)

Add to Collection

Article Biochemical Research Methods

Ion concentration polarization on paper-based microfluidic devices and its application to preconcentrate dilute sample solutions

Ruey-Jen Yang et al.

BIOMICROFLUIDICS (2015)

Add to Collection

Article Biochemical Research Methods

Increasing hybridization rate and sensitivity of DNA microarrays using isotachophoresis

Crystal M. Han et al.

LAB ON A CHIP (2014)

Add to Collection

Article Biochemical Research Methods

Enhancing the lateral-flow immunoassay for detection of proteins using an aqueous two-phase micellar system

Foad Mashayekhi et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2012)

Add to Collection

Article Chemistry, Multidisciplinary

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

Sergio Mendez et al.

LANGMUIR (2010)

Add to Collection

Article Biotechnology & Applied Microbiology

Making it stick: convection, reaction and diffusion in surface-based biosensors

Todd M. Squires et al.

NATURE BIOTECHNOLOGY (2008)

Add to Collection

© Peeref 2019-2024. All rights reserved.