Novel Genes and Key Signaling Molecules Involved in the Repulsive Response of Meloidogyne incognita against Biocontrol Bacteria

The ability of the model organism, Caenorhabditis elegans, to distinguish and escape from pathogenic bacteria has been extensively studied; however, studies on the repulsive response of Meloidogyne incognita are still in their infancy. We have recently demonstrated that biocontrol bacteria induce a repulsive response in M. incognita via two classical signaling pathways. The present study aimed to identify the novel genes and signaling molecules of M. incognita that potentially contribute to its defense reaction. Analysis of the transcriptome data of M. incognita with and without a repulsive response against Bacillus nematocida B16 obtained 15 candidate genes, of which the novel genes Minc3s01748g26034 and Minc3s02548g30585 were found to regulate the aversive behavior of M. incognita, and their functions were further validated. To further confirm the neuronal localization of the two novel genes in M. incognita, in situ hybridization was conducted using the digoxin-labeled probes of ten tag genes, and preferentially profiled the localization of amphid sensory neurons of M. incognita. Analysis of the overviewed neuronal map suggested that Minc3s01748g26034 and Minc3s02548g30585 functioned in ASK/ASI and CEPD/V neurons, respectively. During their interactions, the volatile compounds 3-methyl-butyric acid and 2-methyl-butyric acid produced by the biocontrol bacteria were predicted as the primary signaling molecules that promoted the repulsive behavior of M. incognita against biocontrol bacteria. The findings provided novel insights into the mechanisms underlying the repulsive response of M. incognita that are different from the canonical molecular pathways previously found in C. elegans and can aid in developing novel strategies for controlling root-knot nematodes.

Automated summary

This study identified two novel genes, Minc3s01748g26034 and Minc3s02548g30585, that regulate the aversive behavior of Meloidogyne incognita. In addition, it discovered two volatile compounds produced by biocontrol bacteria that promote the repulsive behavior of M. incognita. These findings provide new insights into the mechanisms underlying the repulsive response of M. incognita and can aid in developing novel strategies for controlling root-knot nematodes.