Journal
VIROLOGY
Volume 589, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2023.109938
Keywords
Avian influenza; Bioinformatics; Bird flu; Highly pathogenic; Introduction; Molecular; Sequence
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We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
We detected and characterized highly pathogenic avian influenza viruses among hunter-harvested wild water-fowl inhabiting western Alaska during September-October 2022 using a molecular sequencing pipeline applied to RNA extracts derived directly from original swab samples. Genomic characterization of 10 H5 clade 2.3.4.4b avian influenza viruses detected with high confidence provided evidence for three independent viral in-troductions into Alaska. Our results highlight the utility and some potential limits of applying molecular pro-cessing approaches directly to RNA extracts from original swab samples for viral research and monitoring.
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