Journal
FEBS OPEN BIO
Volume -, Issue -, Pages -Publisher
WILEY
DOI: 10.1002/2211-5463.13744
Keywords
ATP; glucose-deprivation; hepatocyte; live-cell imaging; mitochondria
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This study visualized the dynamics of metabolites in hepatocytes during recovery from glucose-deprivation. The results suggest that a decrease in mitochondrial ATP concentration is associated with increased protein synthesis rather than glycogen synthesis, activation of urea cycle, and production of reactive oxygen species.
Hepatocytes can switch their metabolic processes in response to nutrient availability. However, the dynamics of metabolites (such as lactate, pyruvate, and ATP) in hepatocytes during the metabolic switch remain unknown. In this study, we visualized metabolite dynamics in primary cultured hepatocytes during recovery from glucose-deprivation. We observed a decrease in the mitochondrial ATP concentration when glucose was administered to hepatocytes under glucose-deprivation conditions. In contrast, there was slight change in the cytoplasmic ATP concentration. A decrease in mitochondrial ATP concentration was associated with increased protein synthesis rather than glycogen synthesis, activation of urea cycle, and production of reactive oxygen species. These results suggest that mitochondrial ATP is important in switching metabolic processes in the hepatocytes.
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