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Summary: A new electrochemical-biosensing strategy using a commercial portable glucose meter (PGM) was developed for the detection of ochratoxin A (OTA). The strategy utilizes an aptamer as the recognition probe and immobilizes it onto the sensing interface. A complementary biotin-modified probe is then hybridized with the aptamer, and biotinylated invertase is introduced onto the sensing system for signal amplification. The glucose produced by the invertase, which is dependent on the amount of OTA, can be conveniently measured with the PGM. This unique PGM-based electrochemical platform shows promising potential in food safety monitoring and has been successfully applied for OTA analysis in rice.
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Summary: A colloidal gold test strip was developed for the detection of hepatitis B virus (HBV) DNA based on CRISPR/Cas13a and RAA technology. It demonstrated high sensitivity and specificity, effectively identifying HBV-infected patients and dynamic plasma samples of patients on antiviral therapy. This study provides a visual and faster alternative to current PCR-based diagnosis for HBV infection.
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