4.5 Article

Comparative investigations into the growth details of Histoplasma capsulatum var farciminosum on four different agar media

Journal

MEDICAL MYCOLOGY
Volume 61, Issue 12, Pages -

Publisher

OXFORD UNIV PRESS
DOI: 10.1093/mmy/myad124

Keywords

Histoplasma capsulatum var farciminosum; EEL; fungal morphology

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This study isolated Histoplasma capsulatum var farciminosum from the eye socket of a horse infected with epizootic equine lymphangitis, and observed and recorded its growth pattern on different solid media and temperatures.
Epizootic equine lymphangitis (EEL) is a chronic fungal disease that affects equids. The causative agent is a dimorphic fungus called Histoplasma capsulatum var farciminosum. Histoplasmacapsulatum var farciminosum field strain 7 (D 2878/2023) isolated from the eye socket of an EEL Ethiopian horse was sub-cultured on four different solid media and incubated at 26 degrees C and 37 degrees C for 6 weeks. Details of growth morphology were recorded and shown in images during 6 weeks of incubation. Histoplasmacapsulatum var farciminosum grew best at 26 degrees C on all four agars, but only on sheep blood agar at 37 degrees C as small, white dry colonies. Histoplasma capsulatum var farciminosum was isolated from the eye socket of an equine epizootic lymphangitis infected Ethiopian horse on Mycosel agar, which was sub-cultured on four different solid media at two different temperatures for 6 weeks to show its growth pattern.

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