4.5 Article

A novel and thermostable phenylalanine dehydrogenase for efficient synthesis of bulky aromatic amino acids and derivatives

Related references

Note: Only part of the references are listed.
Article Biotechnology & Applied Microbiology

Structure-guided steric hindrance engineering of Bacillus badius phenylalanine dehydrogenase for efficient l-homophenylalanine synthesis

Tao Wu et al.

Summary: This study successfully enhanced the efficiency of l-HPA synthesis by engineering PheDH through a structure-guided steric hindrance approach, resulting in the identification of seven superior mutants. The optimal triple-site mutant, V309G/L306V/V144G, showed significantly higher catalytic activity compared to the wild type, achieving the highest substrate conversion rates to date. Docking simulations revealed changes in substrate-binding volume and enzyme tunnels, indicating the potential industrial applications of this mutant for l-HPA synthesis.

BIOTECHNOLOGY FOR BIOFUELS (2021)

Article Chemistry, Physical

Development of a versatile and efficient C-N lyase platform for asymmetric hydroamination via computational enzyme redesign

Yinglu Cui et al.

Summary: This study computationally redesigned aspartase to yield C-N lyases with cross-compatibility of non-native nucleophiles and electrophiles, enabling efficient synthesis of non-canonical amino acids with excellent regioselectivity and enantioselectivity. The process is scalable under industrially relevant protocols and can be easily integrated in cascade reactions, presenting opportunities in synthetic biology.

NATURE CATALYSIS (2021)

Review Chemistry, Applied

Biocatalysis Using Immobilized Enzymes in Continuous Flow for the Synthesis of Fine Chemicals

Matthew P. Thompson et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2019)

Article Chemistry, Multidisciplinary

Biocatalytic Synthesis of Chiral N-Functionalized Amino Acids

Julia F. Hyslop et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Article Biotechnology & Applied Microbiology

Characterization of a Putrescine Transaminase From Pseudomonas putida and its Application to the Synthesis of Benzylamine Derivatives

James L. Galman et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2018)

Article Chemistry, Applied

Single-Biocatalyst Synthesis of Enantiopure D-Arylalanines Exploiting an Engineered D-Amino Acid Dehydrogenase

Fabio Parmeggiani et al.

ADVANCED SYNTHESIS & CATALYSIS (2016)

Article Chemistry, Multidisciplinary

Phenylalanine Ammonia Lyase Catalyzed Synthesis of Amino Acids by an MIO- Cofactor Independent Pathway

Sarah L. Lovelock et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2014)

Article Biochemistry & Molecular Biology

Bacterial Anabaena variabilis phenylalanine ammonia lyase: A biocatalyst with broad substrate specificity

Sarah L. Lovelock et al.

BIOORGANIC & MEDICINAL CHEMISTRY (2014)

Article Biochemistry & Molecular Biology

Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum

Jing Li et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2014)

Article Chemistry, Multidisciplinary

Biocatalysis in Organic Chemistry and Biotechnology: Past, Present, and Future

Manfred T. Reetz

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Review Biochemistry & Molecular Biology

Biocatalytic Synthesis of Chiral Alcohols and Amino Acids for Development of Pharmaceuticals

Ramesh N. Patel

BIOMOLECULES (2013)

Review Multidisciplinary Sciences

Engineering the third wave of biocatalysis

U. T. Bornscheuer et al.

NATURE (2012)

Article Biotechnology & Applied Microbiology

Synthesis of L-homophenylalanine via integrated membrane bioreactor Influence of pH on yield

A. L. Ahmad et al.

BIOCHEMICAL ENGINEERING JOURNAL (2010)

Article Multidisciplinary Sciences

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

Christopher K. Savile et al.

SCIENCE (2010)

Article Biochemistry & Molecular Biology

Discrimination of Aliphatic Substrates by a Single Amino Acid Substitution in Bacillus badius and Bacillus sphaericus Phenylalanine Dehydrogenases

Shinjiro Tachibana et al.

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY (2009)

Article Biotechnology & Applied Microbiology

Phenylalanine dehydrogenase mutants: Efficient biocatalysts for synthesis of non-natural phenylalanine derivatives

Francesca Paradisi et al.

JOURNAL OF BIOTECHNOLOGY (2007)

Article Biochemistry & Molecular Biology

ExPASy: the proteomics server for in-depth protein knowledge and analysis

E Gasteiger et al.

NUCLEIC ACIDS RESEARCH (2003)

Article Chemistry, Medicinal

Substituted N-(3,5-dichlorobenzenesulfonyl)-L-prolyl-phenylalanine analogues as potent VLA-4 antagonists

IE Kopka et al.

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS (2002)