Effect of a Salvia officinalis and Hypericum perforatum mixture on improving memory and cognitive decline

In this study, we explored the effects of herbal mixture containing Sage (Salviaofficinalis) and St. John's Wort (Hypericumperforatum) on cognitive and memory impairment, considering their known cognitive-enhancing properties. We identified 'rosmarinic acid' and 'hyperoside' as marker components influencing memory in Sage and St. John's Wort, respectively. Quantification of these marker compounds in the herbal extracts was performed using high-performance liquid chromatography. Using human neuroblastoma SH-SY5Y cell and murine microglia BV-2 cell, we investigated the effects of the herbal mixture on oxidative stress induced by scopolamine and lipopolysaccharide. Cell viability assays, nitric oxide level quantification, and measurement of cytokines (tumor necrosis factor-alpha, interleukin-1 beta) and catalase expression were performed. The results indicated positive effects of the herbal mixture on these parameters. Furthermore, the herbal mixture oral administered to mouse and induced intentional inflammatory responses and memory impairment by treating them with scopolamine. Cognitive and memory impairment were evaluated using Morris Water Maze and T-maze tests, while cytokines (TNF-a, IL-1 beta) and Catalase in mouse serum were quantified. The group receiving the herbal mixture at a concentration of 10 mg/kg demonstrated significantly reduced TNF-a and IL-1 beta levels, increased Catalase expression, and improved cognitive performance in the Morris Water Maze and T-maze tests. These findings suggest that the intake of the herbal mixture containing Sage (S.officinalis) and St. John's Wort (H.perforatum) may ameliorate cognitive and memory impairment through anti-inflammatory and antioxidant mechanisms.Graphical abstractSage (Salvia officinalis) and St. John's wort (Hypericum perforatum) ethanol extract was mixed in a 1:1 ratio, and the effect of improving memory and cognition was confirmed through cell and animal experiments

Automated summary

This study investigated the effects of a herbal mixture containing Sage and St. John's Wort on cognitive and memory impairment. Marker components were identified and quantified, and the herbal mixture showed positive effects on oxidative stress, cytokine levels, and cognitive performance in both cell and animal experiments.