Trioxidized cysteine in the aging proteome mimics the structural dynamics and interactome of phosphorylated serine

Aging is the primary risk factor for the development of numerous human chronic diseases. On a molecular level, it significantly impacts the regulation of protein modifications, leading to the accumulation of degenerative protein modifications (DPMs) such as aberrant serine phosphorylation (p-Ser) and trioxidized cysteine (t-Cys) within the proteome. The altered p-Ser is linked to abnormal cell signaling, while the accumulation of t-Cys is associated with chronic diseases induced by oxidative stress. Despite this, the potential cross-effects and functional interplay between these two critical molecular factors of aging remain undisclosed. This study analyzes the aging proteome of wild-type C57BL/6NTac mice over 2 years using advanced proteomics and bioinformatics. Our objective is to provide a comprehensive analysis of how t-Cys affects cell signaling and protein structure in the aging process. The results obtained indicate that t-Cys residues accumulate in the aging proteome, interact with p-Ser interacting enzymes, as validated in vitro, and alter their structures similarly to p-Ser. These findings have significant implications for understanding the interplay of oxidative stress and phosphorylation in the aging process. Additionally, they open new venues for further research on the role(s) of these protein modifications in various human chronic diseases and aging, wherein exacerbated oxidation and aberrant phosphorylation are implicated. Trioxidized cysteine, product of cellular prooxidant processes, demonstrates an age-related increase in various mammalian proteomes. These post-translationally modified residues exhibit the capacity to interact with enzymes that mediate phosphorylation signaling, mimicking features of phosphorylated serine residues and likely contributing to the alteration of cell signaling during aging. Additionally, we demonstrate that phosphorylation-linked kinase activity becomes modulated in an oxidation-dependent manner.image

Automated summary

This study provides a comprehensive analysis of the impact of oxidative stress and phosphorylation on the aging process. The results show that trioxidized cysteine residues accumulate in the aging proteome and interact with enzymes involved in phosphorylation signaling, altering their structures in a similar way to phosphorylated serine residues. These findings are significant for understanding the interplay between oxidative stress and phosphorylation in aging, and open new avenues for research on protein modifications in chronic diseases and aging.