☆ 4.8 Article

On the interactions between RNA and titrateable lipid layers: implications for RNA delivery with lipid nanoparticles

NANOSCALE (2023)

Related references

Note: Only part of the references are listed.

Article Chemistry, Multidisciplinary

Advice on describing Bayesian analysis of neutron and X-ray reflectometry

Andrew R. McCluskey et al.

Summary: Due to the availability of modern software and hardware, Bayesian analysis is gaining popularity in neutron and X-ray reflectometry analysis. However, inconsistencies in the representation of probability distributions central to Bayesian methods in the literature may hinder the understandability and replicability of these analyses. In this article, advice is provided on how to report the results of Bayesian analysis in neutron and X-ray reflectometry, with a focus on clear reporting of initial starting conditions, prior probabilities, analysis results, and posterior probabilities. It is believed that this advice, based on the authors' experience, will enhance analytical reproducibility and improve the quality and usability of reflectometry results.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2023)

Add to Collection

Article Biochemistry & Molecular Biology

Ionizable lipids penetrate phospholipid bilayers with high phase transition temperatures: perspectives from free energy calculations

Inna Ermilova et al.

Summary: The study investigated the binding ability of DLin-MC3-DMA to model membranes with different phospholipids and saturation levels. DLin-MC3-DMA showed affinity for the saturated lipid bilayer and aversion to the unsaturated one. The preference for specific membranes was correlated with phase transition temperatures and structural and dynamical properties. Comparisons with ionizable lipids from COVID-19 mRNA vaccines were performed, revealing that ALC-0315 had the strongest binding to the membrane.

CHEMISTRY AND PHYSICS OF LIPIDS (2023)

Add to Collection

Article Chemistry, Multidisciplinary

Development of a high-throughput platform for screening lipid nanoparticles for mRNA delivery

Lili Cui et al.

Summary: This article introduces an automated high-throughput screening platform for selecting novel ionisable lipids and LNPs encapsulating mRNA. The platform allows efficient and precise preparation of LNPs and has been successfully used to screen for novel mRNA LNP leads.

NANOSCALE (2022)

Add to Collection

Article Pharmacology & Pharmacy

Prediction of lipid nanoparticles for mRNA vaccines by the machine learning algorithm

Wei Wang et al.

Summary: The study utilized machine learning algorithms and molecular dynamic modeling to accelerate the development of mRNA vaccine LNPs, demonstrating the feasibility of the predictive model. Experimental validation confirmed the accuracy of the predictive model, providing a new approach for virtual screening of LNP formulations in the future.

ACTA PHARMACEUTICA SINICA B (2022)

Add to Collection

Article Medicine, Research & Experimental

Comparison of DLin-MC3-DMA and ALC-0315 for siRNA Delivery to Hepatocytes and Hepatic Stellate Cells

Francesca Ferraresso et al.

Summary: Ionizable cationic lipids play a crucial role in efficient in vivo delivery of RNA by lipid nanoparticles (LNPs). ALC-0315 LNPs achieved potent siRNA-mediated knockdown of target proteins in hepatocytes and hepatic stellate cells (HSCs) in mice, although markers of liver toxicity were observed at high doses. This study provides insight for the development of ionizable cationic LNP therapeutics with optimal efficacy and minimal toxicity.

MOLECULAR PHARMACEUTICS (2022)

Add to Collection

Article Chemistry, Multidisciplinary

On the Influence of Nucleic Acid Backbone Modifications on Lipid Nanoparticle Morphology

Kevin An et al.

Summary: Nucleic acid therapeutics have made significant progress in treating diseases, but they face challenges such as degradation and clearance. Chemical modifications and lipid nanoparticles can overcome these barriers. This study reveals that chemical modifications can substantially impact the morphology of lipid nanoparticles and enhance their entrapment. It provides insights into the interaction between lipid components and nucleic acids.

LANGMUIR (2022)

Add to Collection

Article Medicine, Research & Experimental

Leveraging Biological Buffers for Efficient Messenger RNA Delivery via Lipid Nanoparticles

Michael I. Henderson et al.

Summary: This study investigates the impact of commonly used biological buffers on the performance and durability of mRNA-LNPs and finds that Tris or HEPES buffers contribute to better stability and transfection efficiency compared to PBS.

MOLECULAR PHARMACEUTICS (2022)

Add to Collection

Article Multidisciplinary Sciences

Payload distribution and capacity of mRNA lipid nanoparticles

Sixuan Li et al.

Summary: The authors developed a method based on multi-laser cylindrical illumination confocal spectroscopy to quantitatively examine the mRNA and lipid contents in LNP formulations at the single-nanoparticle level. They revealed that a commonly referenced benchmark formulation containing DLin-MC3 as the ionizable lipid has mostly 2 mRNAs per loaded LNP and a presence of 40%-80% empty LNPs depending on the assembly conditions.

NATURE COMMUNICATIONS (2022)

Add to Collection

Article Biology

Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration

Manuel J. Carrasco et al.

Summary: The study reports a method that can predict the pKa of lipid nanoparticles (LNPs) from the structure of the ionizable lipid, and investigates the delivery efficiency and factors influencing LNPs both in vitro and in vivo. It was also found that LNPs with more negative charge lead to higher off-target systemic mRNA expression.

COMMUNICATIONS BIOLOGY (2021)

Add to Collection

Review Pharmacology & Pharmacy

Lipid Nanoparticles as Delivery Systems for RNA-Based Vaccines

Basmah N. Aldosari et al.

Summary: RNA-based vaccines have gained increased interest for protection against infectious diseases and cancer immunotherapies. Lipid nanoparticles (LNPs) are being studied as efficient carriers for mRNA delivery due to their easy manufacturing processes, offering promising alternatives to conventional vaccine technologies.

PHARMACEUTICS (2021)

Add to Collection

Article Biophysics

Encapsulation state of messenger RNA inside lipid nanoparticles

Mark L. Brader et al.

Summary: Understanding the structure and microenvironment of mRNA within lipid nanoparticles is crucial for unlocking their biomedical potential. By using the permeating cationic dye thionine as a contrasting agent, cryo-electron microscopy images reveal the location of mRNA within the nanoparticles, suggesting it may be in solvent-filled cavities or associated with lipids.

BIOPHYSICAL JOURNAL (2021)

Add to Collection

Article Biochemistry & Molecular Biology

Cooperativity of α-Synuclein Binding to Lipid Membranes

Katarzyna Makasewicz et al.

Summary: Cooperative binding is a crucial feature in metabolic pathways, signaling, and transport processes, allowing for tight regulation over a narrow concentration range. The study shows strong positive cooperativity of alpha-synuclein binding to phospholipid membranes, indicating that the protein has a higher affinity for a membrane where there is already bound protein, compared to a bare membrane.

ACS CHEMICAL NEUROSCIENCE (2021)

Add to Collection

Review Nanoscience & Nanotechnology

Lipid nanoparticles for mRNA delivery

Xucheng Hou et al.

Summary: Lipid nanoparticle-mRNA formulations have successfully entered clinical use as COVID-19 vaccines, representing a major milestone in mRNA therapeutics. This review discusses the design of lipid nanoparticles for mRNA delivery, emphasizing key points for clinical translation and preclinical studies of lipid nanoparticle-mRNA therapeutics for various diseases.

NATURE REVIEWS MATERIALS (2021)

Add to Collection

Review Pharmacology & Pharmacy

Lipid nanoparticles for nucleic acid delivery: Current perspectives

Eleni Samaridou et al.

ADVANCED DRUG DELIVERY REVIEWS (2020)

Add to Collection

Article Pharmacology & Pharmacy

Manufacturing Considerations for the Development of Lipid Nanoparticles Using Microfluidics

Carla B. Roces et al.

PHARMACEUTICS (2020)

Add to Collection

Article Chemistry, Physical

DOPC versus DOPE as a helper lipid for gene-therapies: molecular dynamics simulations with DLin-MC3-DMA

Inna Ermilova et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2020)

Add to Collection

Review Biochemistry & Molecular Biology

Structural transitions in poly(A), poly(C), poly(U), and poly(G) and their possible biological roles

Margarita I. Zarudnaya et al.

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (2019)

Add to Collection

Article Chemistry, Multidisciplinary

More bang for your buck: Improved use of GPU nodes for GROMACS 2018

Carsten Kutzner et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2019)

Add to Collection

Article Biotechnology & Applied Microbiology

Delivery of mRNA vaccines with heterocyclic lipids increases anti-tumor efficacy by STING-mediated immune cell activation

Lei Miao et al.

NATURE BIOTECHNOLOGY (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Self-Assembled Fluid Phase Floating Membranes with Tunable Water Interlayers

Luke A. Clifton et al.

LANGMUIR (2019)

Add to Collection

Article Biochemistry & Molecular Biology

Barnaba: software for analysis of nucleic acid structures and trajectories

Sandro Bottaro et al.

RNA (2019)

Add to Collection

Article Chemistry, Multidisciplinary

refnx: neutron and X-ray reflectometry analysis in Python

Andrew R. J. Nelson et al.

JOURNAL OF APPLIED CRYSTALLOGRAPHY (2019)

Add to Collection

Article Chemistry, Multidisciplinary

On the role of helper lipids in lipid nanoparticle formulations of siRNA

Jayesh A. Kulkarni et al.

NANOSCALE (2019)

Add to Collection

Article Chemistry, Physical

Structure of Lipid Nanoparticles Containing siRNA or mRNA by Dynamic Nuclear Polarization-Enhanced NMR Spectroscopy

Jasmine Viger-Gravel et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2018)

Add to Collection

Article Biotechnology & Applied Microbiology

A Novel Amino Lipid Series for mRNA Delivery: Improved Endosomal Escape and Sustained Pharmacology and Safety in Non-human Primates

Staci Sabnis et al.

MOLECULAR THERAPY (2018)

Add to Collection

Article Biochemistry & Molecular Biology

Molecular Mechanistic Insights into Drosophila DHX36-Mediated G-Quadruplex Unfolding: A Structure-Based Model

Wei-Fei Chen et al.

STRUCTURE (2018)

Add to Collection

Review Nanoscience & Nanotechnology

Tools for translation: non-viral materials for therapeutic mRNA delivery

Khalid A. Hajj et al.

NATURE REVIEWS MATERIALS (2017)

Add to Collection

Article Biochemistry & Molecular Biology

Supported lipid bilayer platforms to probe cell mechanobiology

Roxanne Glazier et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2017)

Add to Collection

Review Genetics & Heredity

Genome-Wide Analysis of RNA Secondary Structure

Philip C. Bevilacqua et al.

ANNUAL REVIEW OF GENETICS, VOL 50 (2016)

Add to Collection

Review Cell Biology

The new (dis)order in RNA regulation

Aino I. Jaervelin et al.

CELL COMMUNICATION AND SIGNALING (2016)

Add to Collection

Article Chemistry, Physical

Microfluidic Mixing: A General Method for Encapsulating Macromolecules in Lipid Nanoparticle Systems

Alex K. K. Leung et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2015)

Add to Collection

Article Chemistry, Multidisciplinary

Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays

Aleksandra P. Dabkowska et al.

NANO LETTERS (2014)

Add to Collection

Article Instruments & Instrumentation

Mantid-Data analysis and visualization package for neutron scattering and μ SR experiments

O. Arnold et al.

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT (2014)

Add to Collection

Article Biochemistry & Molecular Biology

NKAP is a novel RS-related protein that interacts with RNA and RNA binding proteins

Bhagyashri D. Burgute et al.

NUCLEIC ACIDS RESEARCH (2014)

Add to Collection

Article Multidisciplinary Sciences

Degradable lipid nanoparticles with predictable in vivo siRNA delivery activity

Kathryn A. Whitehead et al.

NATURE COMMUNICATIONS (2014)

Add to Collection

Article Chemistry, Multidisciplinary

Structure of the Parallel Duplex of Poly(A) RNA: Evaluation of a 50 Year-Old Prediction

Nozhat Safaee et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)

Add to Collection

Article Computer Science, Interdisciplinary Applications

A flexible algorithm for calculating pair interactions on SIMD architectures

Szilard Pall et al.

COMPUTER PHYSICS COMMUNICATIONS (2013)

Add to Collection

Article Biotechnology & Applied Microbiology

Biodegradable Lipids Enabling Rapidly Eliminated Lipid Nanoparticles for Systemic Delivery of RNAi Therapeutics

Martin A. Maier et al.

MOLECULAR THERAPY (2013)

Add to Collection

Article Chemistry, Multidisciplinary

Maximizing the Potency of siRNA Lipid Nanoparticles for Hepatic Gene Silencing In Vivo

Muthusamy Jayaraman et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2012)

Add to Collection

Article Chemistry, Physical

Lipid Nanoparticles Containing siRNA Synthesized by Microfluidic Mixing Exhibit an Electron-Dense Nanostructured Core

Alex K. K. Leung et al.

JOURNAL OF PHYSICAL CHEMISTRY C (2012)

Add to Collection

Article Chemistry, Physical

ssRNA base pairing at a bilayer interface can be controlled by the acyl chain order

Agnes Michanek et al.

SOFT MATTER (2012)

Add to Collection

Article Biochemistry & Molecular Biology

RNA and DNA interactions with zwitterionic and charged lipid membranes - A DSC and QCM-D study

Agnes Michanek et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2010)

Add to Collection

Article Biochemical Research Methods

Combinatorial Approach to Determine Functional Group Effects on Lipidoid-Mediated siRNA Delivery

Kerry P. Mahon et al.

BIOCONJUGATE CHEMISTRY (2010)

Add to Collection

Article Biotechnology & Applied Microbiology

Rational design of cationic lipids for siRNA delivery

Sean C. Semple et al.

NATURE BIOTECHNOLOGY (2010)

Add to Collection

Article Multidisciplinary Sciences

Lipid-like materials for low-dose, in vivo gene silencing

Kevin T. Love et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)

Add to Collection

Article Chemistry, Physical

Diffusion in supported lipid bilayers: Influence of substrate and preparation technique on the internal dynamics

C. Scomparin et al.

EUROPEAN PHYSICAL JOURNAL E (2009)

Add to Collection

Article Biochemistry & Molecular Biology

Structural characterization of cationic lipid-tRNA complexes

Regis Marty et al.

NUCLEIC ACIDS RESEARCH (2009)

Add to Collection

Article Biophysics

A Method Improving the Accuracy of Fluorescence Recovery after Photobleaching Analysis

Peter Jonsson et al.

BIOPHYSICAL JOURNAL (2008)

Add to Collection

Article Chemistry, Physical

P-LINCS: A parallel linear constraint solver for molecular simulation

Berk Hess

JOURNAL OF CHEMICAL THEORY AND COMPUTATION (2008)

Add to Collection

Article Chemistry, Physical

GROMACS 4: Algorithms for highly efficient, load-balanced, and scalable molecular simulation

Berk Hess et al.

JOURNAL OF CHEMICAL THEORY AND COMPUTATION (2008)

Add to Collection

Article Biotechnology & Applied Microbiology

A combinatorial library of lipid-like materials for delivery of RNAi therapeutics

Akin Akinc et al.

NATURE BIOTECHNOLOGY (2008)

Add to Collection

Article Chemistry, Physical

Canonical sampling through velocity rescaling

Giovanni Bussi et al.

JOURNAL OF CHEMICAL PHYSICS (2007)

Add to Collection

Article Chemistry, Multidisciplinary

Formation of solid-supported lipid bilayers:: An integrated view

RP Richter et al.

LANGMUIR (2006)

Add to Collection

Article Chemistry, Physical

Structural transitions in polyriboadenylic acid induced by the changes in pH and temperature: Vibrational circular dichroism study in solution and film states

AG Petrovic et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2005)

Add to Collection

Article Chemistry, Multidisciplinary

Cationic lipid saturation influences intracellular delivery of encapsulated nucleic acids

J Heyes et al.

JOURNAL OF CONTROLLED RELEASE (2005)

Add to Collection

Article Chemistry, Physical

Effect of the choice of the pressure coupling method on the spontaneous aggregation of DPPC molecules

RY Patel et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2005)

Add to Collection

Article Biophysics

Direct visualization of asymmetric behavior in supported lipid bilayers at the gel-fluid phase transition

ZV Feng et al.

BIOPHYSICAL JOURNAL (2005)

Add to Collection

Article Chemistry, Physical

Methodological issues in lipid bilayer simulations

C Anézo et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2003)

Add to Collection

Article Biochemistry & Molecular Biology

Efficient encapsulation of antisense oligonucleotides in lipid vesicles using ionizable aminolipids: formation of novel small multilamellar vesicle structures

SC Semple et al.

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES (2001)

Add to Collection

© Peeref 2019-2024. All rights reserved.