4.7 Article

Discovery of a near-infrared fluorescent probe for G-quadruplexes by exploiting the concept of unfolding-intramolecular-aggregation-induced emission

Related references

Note: Only part of the references are listed.
Article Chemistry, Physical

Parallel G-Quadruplex DNA Structures from Nuclear and Mitochondrial Genomes Trigger Emission Enhancement in a Nonfluorescent Nano-aggregated Fluorine-Boron-Based Dye

Marco Deiana et al.

Summary: This study reports the use of parallel DNA guanine quadruplexes (G4s) as supramolecular disaggregating-capture systems capable of activating the fluorescence signal of a zwitterionic fluorine-boron-based dye. The dye selectively disassembles its supramolecular state upon contact with parallel G4s due to its high binding affinity for them compared to nonparallel topologies. This results in a strong and selective disaggregation-induced emission that can be observed by the naked eye and inside cells, making it useful for various affinity-based applications in sensing and imaging systems.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2023)

Article Chemistry, Analytical

Molecular engineering of a wash-free, clover-like G-quadruplex fluorescent probe that exploits a new mechanism of disaggregation-and-binding-induced emission

Xiao-Dong Wang et al.

Summary: In this study, a new principle called disaggregation-and-binding-induced emission (DBIE) was proposed for the design of G4 fluorescent probes, which can overcome the shortcomings of disaggregation-induced emission (DIE) and aggregation-induced emission (AIE) principles. Based on this principle, a wash-free, clover-like fluorescent probe (TCN3) that can detect in vitro or cellular DNA G4s was successfully developed. It is worth noting that this DBIE mechanism can also be used to design promising fluorescent probes targeting other biotargets.

SENSORS AND ACTUATORS B-CHEMICAL (2023)

Article Biochemistry & Molecular Biology

Discovery of a minimalistic DIE-based fluorescent probe for detection of parallel G-quadruplex forms

Yun Dong et al.

Summary: A minimalistic fluorescent probe has been developed in this study, which exhibits selective detection and mapping of parallel topology of cellular RNA G4.

BIOORGANIC CHEMISTRY (2023)

Article Biochemistry & Molecular Biology

Rational design of small-molecules to recognize G-quadruplexes of c-MYC promoter and telomere and the evaluation of their in vivo antitumor activity against breast cancer

Wei Long et al.

Summary: This study reports a new approach of designing small molecules based on a non-selective thiazole orange scaffold to target G4 structures with improved selectivity. The ligands establish multi-site interactions with flanking residues and loops of the G4 motif, and demonstrate antitumor activity in cancer cells.

NUCLEIC ACIDS RESEARCH (2022)

Article Chemistry, Analytical

Detection of G-quadruplex structures with a minimalistic crescent-shaped carbazole-indolium ligand

Jia-Hong Lin et al.

Summary: G-quadruplexes (G4s) are non-canonical DNA or RNA secondary structures implicated in the regulation of key biological processes. This study designed and synthesized a fluorescent ligand KN-1, which can detect and stabilize RNA G4 structures in live cells. It provides a promising tool for investigating the functions of specific RNA G4s in living cells.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Article Chemistry, Analytical

Thionation toward High-Contrast ACQ-DIE Probes by Reprogramming the Aqueous Segregation Behavior: Enlightenment from a Sulfur-Substituted G-Quadruplex Ligand

Jing Huang et al.

Summary: Small-molecule-based fluorescent chemosensors are powerful tools in analytical chemistry, but they often suffer from aggregation-caused quenching (ACQ) in water. In this study, we proposed that sulfur substitution on a molecule could lead to an evolved ACQ-DIE probe. By modifying a G-quadruplex (G4) ligand to its thionation version, we obtained a probe called CTQ, which exhibited improved fluorescence performance and selective binding to G4 structures. CTQ has the potential to be used as a probe for accurate analysis of G4-related assays, particularly for distinguishing virus variants.

ANALYTICAL CHEMISTRY (2022)

Article Chemistry, Analytical

A subtle structural change in a near-infrared fluorescent skeleton remolds its sensing properties for G-quadruplexes

Ming-Hao Hu

Summary: In this study, a promising NIR probe named VIN-2 was developed for real-time visualization of G-quadruplex structures in live cells. VIN-2 was proven to bind to terminal guanine tetrads and emit significant fluorescence. It also showed potential for monitoring mitochondrial G4 folding dynamics in real time.

SENSORS AND ACTUATORS B-CHEMICAL (2022)

Article Chemistry, Analytical

Pyrazine-based G-quadruplex fluorescent probes: Transformation between aggregation-induced emission and disaggregation-induced emission via slight variations in structures

Ming-Hao Hu

Summary: The study designed two pyrazine-based G4 fluorescent probes based on the AIE or DIE principles and investigated the fluorescence-triggering mechanisms upon binding to G4s; one probe was selected for further studies, demonstrating its ability to selectively sense various G4s; By exploring the binding modes, it was revealed that G4s could dissociate the probe aggregate and trigger its fluorescence through a specific end-stacking mode.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

Article Chemistry, Multidisciplinary

Monitoring and Modulating mtDNA G-Quadruplex Dynamics Reveal Its Close Relationship to Cell Glycolysis

Xiu-Cai Chen et al.

Summary: The research found that mtDNA G4s are prevalent only in endothelial and cancer cells that rely on glycolysis for energy production, and promoting mtDNA G4 folding can affect gene activation and enhancement of glycolysis, allowing MitoISCH to accumulate in tumors and label them in vivo.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Article Chemistry, Medicinal

Molecular Recognition and Imaging of Human Telomeric G-Quadruplex DNA in Live Cells: A Systematic Advancement of Thiazole Orange Scaffold To Enhance Binding Specificity and Inhibition of Gene Expression

Wei Long et al.

Summary: The series of fluorescent ligands constructed from thiazole orange scaffold were investigated for their interactions with G-quadruplex structures and antitumor activity. Compound 3 showed excellent specificity towards telomere G4-DNA, with higher affinity compared to other nucleic acids. The findings support that compound 3 may be telomere G4-DNA specific and offer insights for anticancer drug design.

JOURNAL OF MEDICINAL CHEMISTRY (2021)

Article Chemistry, Analytical

Development of a near-infrared fluorescent ligand that visualizes and stabilizes G-quadruplexes by decorating the triphenylamine scaffold

Xiaodong Wang et al.

Summary: G-quadruplex structures are enriched in cancer-driving genes and considered potential targets for cancer therapy. Two pi-extended compounds were synthesized to visualize and stabilize G4s, with TAIN-2 showing promising ability to bind and stabilize G4s in TNBC cells, suggesting its potential as a dual-function agent for diagnosis and therapy.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

Article Chemistry, Analytical

Molecular engineering of a near-infrared fluorescent ligand for tracking mitochondrial DNA G-quadruplexes

Ming-Hao Hu

Summary: This study successfully designed a near-infrared fluorescence probe IZIN-1 through engineering, which can track the formation of mtDNA G4 in live cells. This provides a promising example of designing a fluorescent probe targeting mtDNA G4, and offers a feasible chemical tool for investigating mtDNA G4s in live cells.

ANALYTICA CHIMICA ACTA (2021)

Article Chemistry, Multidisciplinary

A Light-up Logic Platform for Selective Recognition of Parallel G-Quadruplex Structures via Disaggregation-Induced Emission

Marco Deiana et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

Review Cell Biology

The regulation and functions of DNA and RNA G-quadruplexes

Dhaval Varshney et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2020)

Article Chemistry, Multidisciplinary

Single-molecule visualization of DNA G-quadruplex formation in live cells

Marco Di Antonio et al.

NATURE CHEMISTRY (2020)

Review Chemistry, Analytical

Fluorescent J-aggregates of cyanine dyes: basic research and applications review

Julia L. Bricks et al.

METHODS AND APPLICATIONS IN FLUORESCENCE (2018)

Article Chemistry, Multidisciplinary

Tracking the Dynamic Folding and Unfolding of RNA G-Quadruplexes in Live Cells

Xiu-Cai Chen et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Review Chemistry, Multidisciplinary

G-Quadruplex: A Regulator of Gene Expression and Its Chemical Targeting

Tian Tian et al.

Article Spectroscopy

Intramolecular aggregation and optical limiting properties of triazine-linked mono-, bis- and tris-phthalocyanines

Jun Chen et al.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2015)

Article Chemistry, Multidisciplinary

Solution Structure of a 2:1 Quindoline-c-MYC G-Quadruplex: Insights into G-Quadruplex-Interactive Small Molecule Drug Design

Jixun Dai et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

Article Chemistry, Multidisciplinary

Fluorescent sensors for specific RNA: A general paradigm using chemistry and combinatorial biology

Brian A. Sparano et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2007)