Generation of an NSD2-deficient human embryonic stem cell line using CRISPR/Cas9 technology

NSD2 is a histone methyltransferase (HMT) and is involved in the epigenetic regulation of hematopoiesis and hematological cancers. To understand and illustrate the precise roles of NSD2 in hematopoietic development, here we constructed a human embryonic stem cell (hESC) line with knockout of NSD2 using CRISPR/Cas9mediated gene targeting. The cell line maintained typical stem cell morphology and normal karyotype. Furthermore, the pluripotency of the cell line was evidenced by high expression level of pluripotency genes and differentiation potential into three germ layers. The cell line provides a good model for studying roles of NSD2 in embryonic development, especially hematopoiesis.

Automated summary

In this study, a human embryonic stem cell line with knockout of NSD2 was constructed using CRISPR/Cas9-mediated gene targeting. This cell line serves as a good model for studying the roles of NSD2 in embryonic development, particularly in hematopoiesis.