Robust rehabilitation of anammox system by granular activated carbon under long-term starvation stress: Microbiota restoration and metabolic reinforcement

This article investigates the buffering capacity and recovery-enhancing ability of granular activated carbon (GAC) in a starved (influent total nitrogen: 20 mg/L) anaerobic ammonium oxidation (anammox) reactor. The findings revealed that anammox aggregated and sustained basal metabolism with shorter performance recovery lag (6 days) and better nitrogen removal efficiency (84.9 %) due to weak electron-repulsion and abundance redox-active groups on GAC's surface. GAC-supported enhanced extracellular polymeric substance secretion aided anammox in resisting starvation. GAC also facilitated anammox bacterial proliferation and expedited the restoration of anammox microbial community from a starved state to its initial-level. Metabolic function analyses unveiled that GAC improved the expression of genes involved in amino acid metabolism and sugar-nucleotide biosynthesis while promoted microbial cross-feeding, ultimately indicating the superior potential of GAC in stimulating more diverse metabolic networks in nutrient-depleted anammox consortia. This research sheds light on the microbial and metabolic mechanisms underlying GAC-mediated anammox system in low-substrate habitats.

Automated summary

This article investigates the buffering capacity and recovery-enhancing ability of granular activated carbon (GAC) in a starved anaerobic ammonium oxidation (anammox) reactor. The findings reveal that GAC's surface characteristics enable faster recovery and improved nitrogen removal efficiency. Additionally, GAC promotes extracellular polymeric substance secretion, bacterial proliferation, and microbial community restoration. Metabolic function analysis demonstrates that GAC stimulates more diverse metabolic networks.