Journal
METHODSX
Volume 12, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.mex.2023.102492
Keywords
Sleep disturbance; Sleep/wake cycles; Zebrafish larvae; Video-tracking technologies
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This article presents a protocol for assessing sleep/wake cycles in zebrafish larvae using video-tracking technologies and demonstrates its suitability for studying the effect of environmental pollutants on sleep.
Zebrafish larvae are a model organism increasingly used in the study of the effect of neuroactive chemicals on vertebrate sleep/wake cycles. Sleep disturbances have a negative impact on mood, cognition and overall health. Here we present a protocol to assess over 24 h sleep/wake cycles in zebrafish larvae subjected to 12 h light/dark periods in 48-well plates, using video-tracking technologies. The protocol can be used to determine if the exposure to environmental pollutants or drugs can lead to sleep disturbances. The results on the effect of the tire rubber-derived 6PPDquinone on zebrafish sleep/wake cycles presented here demonstrate the suitability of using this protocol in fish neurotoxicity studies. This protocol provides a new relevant tool to be used in the pharmacology and toxicology fields.
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