4.7 Article

Insight into the interaction mechanism between mulberry polyphenols and β-lactoglobulin

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Identification of binding sites for Tartary buckwheat protein-phenols covalent complex and alterations in protein structure and antioxidant properties

Dongze Li et al.

Summary: The effects of structure, multiple binding sites and antioxidant property on Tartary buckwheat protein-phenols covalent complex were investigated. Four kinds of phenols (rutin, quercetin, kaempferol, and myricetin) were identified to successfully bind to the protein. The binding of phenols can change the secondary/tertiary structure of the protein. The particle distribution showed that the binding of phenols reduced the particle size, but cross-linking occurred when the bound phenol content was too high. Proteomics analysis revealed that only rutin, quercetin, and myricetin can covalently bind to the protein, and four peptides covalently bound to phenols were identified. The protein-phenol covalent complexes showed higher antioxidant activity than pure protein, indicating their potential as functional components in the food field.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

Article Food Science & Technology

Conformational changes and functional properties of soy lipophilic protein-epicatechin complexes formed by non-covalent interaction

Bingyu Sun et al.

Summary: Soy lipoprotein (SLP) is the major component of soy protein isolate and has the potential to reduce blood cholesterol and triglyceride levels. This study examined the interaction between SLP and epicatechin (EC) and their impact on protein structure, binding mode, physicochemical properties, and antioxidant activities.

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY (2023)

Review Chemistry, Medicinal

The phenolic components extracted from mulberry fruits as bioactive compounds against cancer: A review

Mohd Younus Wani et al.

Summary: The phenolic compounds in white mulberry and black mulberry play a significant role in cancer prevention, but due to a lack of recognition and consistent testing techniques, they have not been formally or scientifically recognized.

PHYTOTHERAPY RESEARCH (2023)

Article Chemistry, Applied

A model study on the site-specificity of (-)-epicatechin-induced reactions in β-lactoglobulin by high-resolution mass spectrometry in combination with bioinformatics

Amelie Boersig et al.

Summary: Polyphenol-protein reactions in beta-lactoglobulin model solutions incubated with (-)-epicatechin were analyzed using microLC-timsTOF Pro-MS/MS combined with bioinformatics strategies. The addition of (-)-epicatechin resulted in changes in tryptic peptide profiles and covalent bond formation between (-)-epicatechin o-quinones and beta-LG. The study also identified significant oxidation promoted by the incubation of beta-LG with (-)-epicatechin, and the presence of (-)-epicatechin o-quinone in milk chocolate samples. This study has important implications for comprehensive food profiling strategies.

FOOD CHEMISTRY (2023)

Article Chemistry, Applied

Storage stability and multi-spectroscopy analysis of the ternary complex induced by mulberry anthocyanin extract interacting with whey protein isolate and rutin under acidic conditions

Yong Cheng et al.

Summary: In this study, the researchers investigated the mechanisms of stabilizing effects of whey protein isolate (WPI) and rutin (Ru) on the storage of mulberry anthocyanin extract (MAE) solution at pH 3.6. The results showed that the combination of WPI and Ru reduced the degradation rate of anthocyanin by 16.2% and 18.3% compared to using them individually. The analysis indicated the formation of binary and ternary complexes involving ll-lactoglobulin (ll-LG), cyanidin-3-Oglucoside (C3G), and Ru. The study provides a foundation for using ternary complexes to enhance the storage stability of anthocyanins.

FOOD HYDROCOLLOIDS (2023)

Article Chemistry, Applied

New insights into the pH dependence of anthocyanin-protein interactions by a case study of cyanidin-3-O-glucoside and bovine serum albumin

Peiqing Yang et al.

Summary: This study investigated the pH-dependence of anthocyanin-protein binding mechanisms using cyandin-3-O-glucoside (C3G) and bovine serum albumin (BSA). The results showed that the binding affinity between C3G and BSA was highest at pH 7, followed by pH 5 and pH 3. Molecular dynamics simulation revealed that electrostatic interactions played a crucial role in the binding mechanisms. Anionic quinoidal bases, the most common C3G forms at pH 7, had the highest binding affinity with BSA due to hydrogen bonding with surrounding amino acids.

FOOD HYDROCOLLOIDS (2023)

Article Food Science & Technology

Impact of the solubility of phenolic compounds from Highland Barley (Hordeum vulgare L.) on their antioxidant property and protein binding affinity

Wangyan Qin et al.

Summary: The solubility of phenolic compounds in highland barley was found to affect their antioxidant property and protein binding affinity. Water-soluble phenolic compounds showed strong antioxidant activity, while acetone-soluble phenolic compounds were positively correlated with protein binding affinity. Additionally, water-insoluble phenolic compounds could lower starch digestibility and postprandial glycemia.

LWT-FOOD SCIENCE AND TECHNOLOGY (2023)

Review Food Science & Technology

Phenolic-protein interactions: insight from in-silico analyses - a review

Fereidoon Shahidi et al.

Summary: Phenolic compounds interact with proteins, altering their structure and properties, and have potential benefits to human health and the food industry. They form complexes via covalent linkages and non-covalent interactions, and the concentration, molecular weight, structure, ions/cofactors, and system conditions determine the complex's precipitation or solubilization. Molecular docking and simulation studies of phenolic-protein interactions allow for a better understanding of these reactions and the development of novel food ingredients and additives.

FOOD PRODUCTION PROCESSING AND NUTRITION (2023)

Article Food Science & Technology

Changes of phenolic profile and antioxidant activity during cold storage of functional flavored yogurt supplemented with mulberry pomace

Huaxin Du et al.

Summary: This study investigated the changes in phenolic compounds and antioxidant activity of functional flavored yogurt supplemented with mulberry pomace during cold storage. The addition of mulberry pomace significantly increased the content of phenolic compounds and antioxidant activity of the yogurt products. The antioxidant activity of the fortified yogurts increased gradually during cold storage, showing the potential of mulberry pomace as a natural antioxidant for functional dairy products.

FOOD CONTROL (2022)

Article Chemistry, Applied

Antioxidant activity, stability, in vitro digestion and cytotoxicity of two dietary polyphenols co-loaded by β-lactoglobulin

Yongfang Ren et al.

Summary: The study investigated the synergistic beneficial effects of multiple dietary polyphenols, as well as the potential improvements due to protein encapsulation. Various techniques were used to analyze the interactions between procyanidin B2 (PB2) and/ or dihydromyricetin (DMY) with 13-lactoglobulin (13-LG), optimizing antioxidant activity and studying stability and bioaccessibility in various systems. The findings provide theoretical support for developing functional foods and beverages with enhanced properties for promoting human health and preventing diseases.

FOOD CHEMISTRY (2022)

Article Biochemistry & Molecular Biology

How Do Phenolic Acids Change the Secondary and Tertiary Structure of Gliadin? Studies with an Application of Spectroscopic Techniques

Renata Welc et al.

Summary: The effect of selected phenolic acids on the molecular organization of gliadins was investigated. The smaller hydroxybenzoic acids incorporated into spaces between polypeptide chains and formed hydrogen bonds, leading to aggregation. Syringic acids could incorporate into hydrophobic pockets of protein. On the other hand, the larger and stiffer hydroxycinnamic acids separated polypeptide chains and caused gliadin disaggregation.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

Article Chemistry, Applied

Different interactions driving the binding of soy proteins (7S/11S) and flavonoids (quercetin/rutin): Alterations in the conformational and functional properties of soy proteins

Yijia Jia et al.

Summary: The purpose of this research was to compare the interactions between bioactive flavonoids (quercetin and rutin) and two predominant soy proteins (beta-conglycinin and glycinin), and the structural and functional properties of their complexes. The study found that the binding affinities of quercetin and rutin towards different soy proteins were structure-dependent. Both quercetin and rutin altered the protein's secondary structure and enhanced its antioxidant capacity.

FOOD CHEMISTRY (2022)

Article Chemistry, Applied

Effect of the non-covalent and covalent interactions between proteins and mono- or di-glucoside anthocyanins on β-lactoglobulin-digestibility

Ibrahim Khalifa et al.

Summary: This study found that C3G/R can enhance the digestibility of β-Lg, with C3R showing better effects than C3G and covalent interactions being more effective than non-covalent ones. In addition, C3G/R can also improve the antioxidant capacity of digested peptides.

FOOD HYDROCOLLOIDS (2022)

Article Food Science & Technology

Effects of different phenolic compounds on the interfacial behaviour of casein and the action mechanism

Lin Chen et al.

Summary: The effects of different phenolic compounds on the interfacial behavior of casein were investigated, and the mechanism of action was further explored. Phenolic compounds were found to bind and form complexes with casein, improving its emulsifying and foaming stabilities. The structure characteristics of phenolic compounds significantly influenced their interactions with casein and its interfacial behavior.

FOOD RESEARCH INTERNATIONAL (2022)

Article Chemistry, Applied

Study on the mechanism of non-covalent interaction between rose anthocyanin extracts and whey protein isolate under different pH conditions

Yun Wang et al.

Summary: This study investigated the non-covalent interaction mechanism between rose anthocyanin extract (RAEs) and whey protein isolate (WPI) and compared it with the interaction mechanism between pure anthocyanin (PC) and WPI. The results showed that RAEs and WPI had non-covalent interactions under different pH conditions, with hydrogen bonds and van der Waals forces playing a role. Interestingly, PC and WPI also exhibited non-covalent interactions, with hydrogen bonds and van der Waals forces as the driving forces. Additionally, changes in WPI's secondary structure were observed in both RAEs and PC.

FOOD CHEMISTRY (2022)

Article Chemistry, Applied

Formation of protein-anthocyanin complex induced by grape skin extracts interacting with wheat gliadins: Multi-spectroscopy and molecular docking analysis

Ziqi Guo et al.

Summary: The interaction between grape skin anthocyanin extract and gliadin was investigated, and it was found that anthocyanins could effectively form complexes with gliadin and change its conformational properties.

FOOD CHEMISTRY (2022)

Article Chemistry, Applied

Deciphering the non-covalent binding patterns of three whey proteins with rosmarinic acid by multi-spectroscopic, molecular docking and molecular dynamics simulation approaches

Yingcong Lu et al.

Summary: The binding between rosmarinic acid (RA) and three whey proteins was investigated in this study. The results showed that the binding occurred through fluorescence quenching, driven by hydrophobic effects. The binding also caused changes in the hydrophobic microenvironment of the proteins.

FOOD HYDROCOLLOIDS (2022)

Article Food Science & Technology

The effects of conjugation of walnut protein isolate with polyphenols on protein solubility, antioxidant activity, and emulsifying properties

Xuan Huang et al.

Summary: This study investigated the influence of ellagic acid (EA) or epigallocatechin-3gallate (EGCG) conjugation on the solubility and emulsifying properties of alkaline-extracted walnut protein isolate (AWPI). The results showed that the polyphenol-conjugated AWPI had improved solubility, enhanced antioxidant capacity, and greater emulsion stability.

FOOD RESEARCH INTERNATIONAL (2022)

Article Food Science & Technology

Pea protein based nanocarriers for lipophilic polyphenols: Spectroscopic analysis, characterization, chemical stability, antioxidant and molecular docking

Xiaoge Zhang et al.

Summary: The research successfully encapsulated lipophilic polyphenols CUR, QUE, and RES into PPI nanocarriers with different loading capacities, without changing the secondary structure of PPI. Results from FTIR, DSC, and XRD confirmed the transformation of polyphenols from crystalline to amorphous state after combination with PPI. PPI effectively shielded polyphenols from UV light and thermal treatment, and improved their antioxidant activity.

FOOD RESEARCH INTERNATIONAL (2022)

Article Chemistry, Applied

Binding of β-lactoglobulin to three phenolics improves the stability of phenolics studied by multispectral analysis and molecular modeling

Shanying Zhang et al.

Summary: This study found that embedding ferulic acid, quercetin, and vanillic acid into bovine beta-lactoglobulin can improve their thermostability and photostability in food. Fluorescence experiments showed that ferulic acid and quercetin exhibited typical static quenching, while vanillic acid caused fluorescence sensitization of beta-lactoglobulin. Furthermore, these phenolics changed the secondary structure of beta-lactoglobulin and played a role through Van der Waals forces and hydrogen bonds. The thermal and photostability of ferulic acid, quercetin, and vanillic acid were significantly enhanced by binding to beta-lactoglobulin, and they demonstrated good inhibitory abilities against AGEs.

FOOD CHEMISTRY-X (2022)

Article Chemistry, Applied

Study on the interaction mechanism of purple potato anthocyanins with casein and whey protein

Shengxiang Gong et al.

Summary: Investigating the interaction mechanism of casein and whey protein with anthocyanins from purple potato flour revealed that casein had a stronger binding affinity with anthocyanins, the binding was spontaneous, and it affected the conformational structures of the proteins.

FOOD HYDROCOLLOIDS (2021)

Review Food Science & Technology

The interactions between anthocyanin and whey protein: A review

Shuai Ren et al.

Summary: This review summarizes the interactions between anthocyanins and whey protein under different conditions, discussing the impact of different chemical structures and conformation changes. The structural changes in whey protein can affect its binding affinity with anthocyanins, mainly through hydrophobic interactions.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2021)

Article Chemistry, Applied

Analysis of the interaction between cyanidin-3-O-glucoside and casein hydrolysates and its effect on the antioxidant ability of the complexes

Zhucheng Yin et al.

Summary: The study found that the interaction between cyanidin-3-O-glucoside and casein or casein hydrolysates is mediated by different forces, forming hydrogen bonds or hydrophobic interactions. After binding with cyanidin-3-O-glucoside, a slight change in the secondary structure of casein/casein hydrolysates was observed, leading to the formation of larger particles. The antioxidant activity assessments showed a synergistic or antagonistic effect between casein/casein hydrolysates and cyanidin-3-O-glucoside.

FOOD CHEMISTRY (2021)

Article Chemistry, Applied

Carboxymethyl tara gum-lactoferrin complex coacervates as carriers for vitamin D3: Encapsulation and controlled release

Monique Barreto Santos et al.

Summary: This study developed a controlled release system of coacervated complexes involving lactoferrin and carboxymethyl tara gum for encapsulation of vitamin D-3. The optimized pH and LF:CMTG ratio showed ideal complex formation conditions, resulting in high encapsulation efficiency and improved thermal stability of the vitamin. In vitro simulations demonstrated effective release and bioaccessibility of the encapsulated vitamin in the intestine. The formation of complexes between LF and CMTG enabled the encapsulation of lipophilic bioactive compounds and promoted controlled release during gastrointestinal simulation.

FOOD HYDROCOLLOIDS (2021)

Article Agriculture, Multidisciplinary

Effects of (-)-Epigallocatechin-3-gallate on the Functional and Structural Properties of Soybean Protein Isolate

Yaohui You et al.

Summary: In this study, soy protein isolate (SPI) was noncovalently modified by (-)-epigallocatechin-3-gallate (EGCG), leading to an improvement in its foaming, emulsifying, and antioxidant properties. The interaction between SPI and EGCG was found to involve hydrophobic force and hydrogen bonds, resulting in looser SPI structure and more active groups exposed. This modification process enhanced the functional properties of SPI significantly.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021)

Article Chemistry, Applied

Effects of different dietary polyphenols on conformational changes and functional properties of protein-polyphenol covalent complexes

Xiangju Liu et al.

Summary: Conjugates of whey protein isolate (WPI) and four polyphenols were prepared through free-radical grafting in this study. The conjugation process significantly enhanced the antioxidant properties and thermal stabilities of polyphenols, while reducing the surface hydrophobicity of the complexes.

FOOD CHEMISTRY (2021)

Article Chemistry, Applied

Conformational changes and functional properties of whey protein isolate-polyphenol complexes formed by non-covalent interaction

Yueyue Meng et al.

Summary: The study evaluated the non-covalent modifications of whey protein isolate with gallic acid, chlorogenic acid and epigallocatechin gallate. The binding with these phenolic compounds led to structural alterations in the protein complexes and improved solubility, foaming and emulsifying capacities. The research suggests the potential application of these polyphenols to enhance the functional properties of whey protein isolate in food industries.

FOOD CHEMISTRY (2021)

Article Food Science & Technology

Characterization of Soybean Protein Isolate-Food Polyphenol Interaction via Virtual Screening and Experimental Studies

Le Ao et al.

Summary: The study established a molecular docking and virtual screening method to investigate the interaction between soybean protein isolate (SPI) and polyphenols, with results showing that the binding pattern is highly dependent on the detailed structure of polyphenols. Flavonoids were found to exhibit higher binding capacity than phenolic acids, with hydrogen bonding and hydrophobic interactions being the dominant forces in SPI-polyphenol binding. Cyanidin chloride demonstrated the highest apparent binding constant among the top-ranking polyphenols.

FOODS (2021)

Review Chemistry, Physical

Assembled milk protein nano-architectures as potential nanovehicles for nutraceuticals

Chuan-he Tang

Summary: The article comprehensively reviews the current research status of utilizing milk proteins as nano-carriers for encapsulating and delivering hydrophobic nutraceuticals, including the composition, structure, assembly behavior, and coassembly with other components of milk proteins for nano-architectures formation.

ADVANCES IN COLLOID AND INTERFACE SCIENCE (2021)

Article Agriculture, Dairy & Animal Science

Effects of mulberry pomace on physicochemical and textural properties of stirred-type flavored yogurt

Huaxin Du et al.

Summary: The study demonstrated that adding mulberry pomace to stirred yogurt can improve the physicochemical and textural properties of the product. Mulberry pomace has great potential for use in dairy products due to its ability to enhance color, acidity, and phytochemical content, as well as improve texture.

JOURNAL OF DAIRY SCIENCE (2021)

Article Chemistry, Physical

Perturbations in the photophysical properties of isoxazole derivative of curcumin up on interaction with different anionic, cationic and non-ionic surfactants

Manisha Sharma et al.

Summary: Surfactants are organized assemblies used as models for biomembranes and solubilizing agents for hydrophobic drug molecules. Isoxazole derivative of curcumin (IOC) has limited biological efficacy due to low aqueous solubility. Different supramolecular confined environments are used to enhance aqueous solubility and study perturbations in photophysical properties of IOC. Three types of surfactants (cationic, anionic, non-ionic) interact with IOC, showing successful encapsulation and stable micellar systems. Salt concentration has no effect on photophysics of IOC. This research opens new opportunities for designing novel drug formulations of IOC.

JOURNAL OF MOLECULAR LIQUIDS (2021)

Article Food Science & Technology

Comparison of non-covalent binding interactions between three whey proteins and chlorogenic acid: Spectroscopic analysis and molecular docking

Yuanyuan Zhang et al.

Summary: In this study, non-covalent binding interactions between three whey proteins and chlorogenic acid were investigated using spectroscopic analysis and molecular docking. The results revealed the contribution of hydrophobic force, different binding affinities and energy transfer probabilities among the proteins. The modeling and docking results suggested the involvement of hydrophobic interaction and hydrogen bond in the formation of molecular nano complexes.

FOOD BIOSCIENCE (2021)

Article Biochemistry & Molecular Biology

Covalent and non-covalent interactions of cyanidin-3-O-glucoside with milk proteins revealed modifications in protein conformational structures, digestibility, and allergenic characteristics

Qiaozhi Zhang et al.

Summary: This study systematically investigated the interactions between cyanidin-3-O-glucoside and two major milk proteins, demonstrating that covalent complexes reduce protein digestibility while exhibiting antioxidant properties and lower allergenicity compared to their non-covalent counterparts.

FOOD & FUNCTION (2021)

Article Biochemistry & Molecular Biology

Stabilization of black rice anthocyanins by self-assembled silk fibroin nanofibrils: Morphology, spectroscopy and thermal protection

Zhen Ma et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Review Food Science & Technology

Bioactivity of dietary polyphenols: The role of metabolites

Simon Vlad Luca et al.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2020)

Article Spectroscopy

Interaction between wheat gliadin and quercetin under different pH conditions analyzed by multi-spectroscopy methods

Qiming Wang et al.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY (2020)

Article Biochemistry & Molecular Biology

Binding Interaction of Juglone with Lysozyme: Spectroscopic Studies Aided by In Silico Calculations

Saumen Saha et al.

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY (2019)

Article Biochemistry & Molecular Biology

Comparative studies of interaction of β-lactoglobulin with three polyphenols

Jinhui Xu et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Review Food Science & Technology

The Role of Dietary Phenolic Compounds in Protein Digestion and Processing Technologies to Improve Their Antinutritive Properties

Tanja D. Cirkovic Velickovic et al.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2018)

Article Chemistry, Applied

Physical bonding between sunflower proteins and phenols: Impact on interfacial properties

Dimitris Karefyllakis et al.

FOOD HYDROCOLLOIDS (2017)

Article Chemistry, Applied

Interaction of cyanidin-3-O-glucoside with three proteins

Tang Lin et al.

FOOD CHEMISTRY (2016)

Article Plant Sciences

Selecting optimum protein nano-carriers for natural polyphenols using chemoinformatics tools

AbdelKader A. Metwally et al.

PHYTOMEDICINE (2016)

Article Food Science & Technology

Impact of calcium on the interactions between epigallocatechin-3-gallate and β-lactoglobulin

Valerie Carnovale et al.

FOOD RESEARCH INTERNATIONAL (2015)

Article Agriculture, Dairy & Animal Science

Conformational changes of β-lactoglobulin induced by shear, heat, and pH-Effects on antigenicity

Toheder Rahaman et al.

JOURNAL OF DAIRY SCIENCE (2015)

Review Food Science & Technology

A review on protein-phenolic interactions and associated changes

Tugba Ozdal et al.

FOOD RESEARCH INTERNATIONAL (2013)

Review Food Science & Technology

Interactions between Polyphenols and Macromolecules: Quantification Methods and Mechanisms

C. Le Bourvellec et al.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2012)

Article Chemistry, Applied

Milk β-lactoglobulin complexes with tea polyphenols

C. D. Kanakis et al.

FOOD CHEMISTRY (2011)

Article Chemistry, Physical

Fluorescence quenching and ligand binding: A critical discussion of a popular methodology

Marco van de Weert et al.

JOURNAL OF MOLECULAR STRUCTURE (2011)

Article Biochemical Research Methods

Fluorescence Quenching to Study Protein-ligand Binding: Common Errors

Marco van de Weert

JOURNAL OF FLUORESCENCE (2010)

Article Agriculture, Multidisciplinary

Interaction of different polyphenols with bovine serum albumin (BSA) and human salivary α-amylase (HSA) by fluorescence quenching

Susana Soares et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2007)

Article Chemistry, Analytical

Isothermal titration calorimetry study of epicatechin binding to serum albumin

Richard A. Frazier et al.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2006)