Journal
ANNALS OF THE AMERICAN THORACIC SOCIETY
Volume 20, Issue 1, Pages 67-74Publisher
AMER THORACIC SOC
DOI: 10.1513/AnnalsATS.202204-314OC
Keywords
inflammation; biomarkers; sputum; lung function; bronchiectasis
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In this study, we investigated the relationship between sputum inflammation measurements, age, lung function, bronchiectasis, airway infection, and ultrastructural defects in children with primary ciliary dyskinesia (PCD). The results showed that elevated concentrations of sputum proteases and cytokines were associated with impaired lung function and structural damage, suggesting that sputum inflammatory measurements could serve as biomarkers in PCD.
Rationale: The role of airway inflammation in disease pathogenesis in children with primary ciliary dyskinesia (PCD) is poorly understood. Objectives: We investigated relationships between sputum inflammation measurements, age, lung function, bronchiectasis, airway infection, and ultrastructural defects in children with PCD. Methods: Spontaneously expectorated sputum was collected from clinically stable children and adolescents with PCD ages 6 years and older participating in a multicenter, observational study. Sputum protease and inflammatory cytokine concentrations were correlated with age, lung function, and chest computed tomography measures of structural lung disease, whereas differences in concentrations were compared between ultrastructural defect categories and between those with and without detectable bacterial infection. Results: Sputum from 77 children with PCD (39 females [51%]; mean [standard deviation] age, 13.9 [4.9] yr; mean [standard deviation] forced expiratory volume in 1 second [FEV1]% predicted, 80.8 [20.5]) was analyzed. Sputum inflammatory marker measurements, including neutrophil elastase activity, IL-1 beta (interleukin-1b), IL-8, and TNF-alpha (tumor necrosis factor alpha) concentrations, correlated positively with age, percentage of bronchiectasis, and percentage of total structural lung disease on computed tomography, and negatively with lung function. Correlations between neutrophil elastase concentrations and FEV1% predicted and percentage of bronchiectasis were 20.32 (95% confidence interval, 20.51 to 20.10) and 0.46 (0.14 to 0.69), respectively. Sputum neutrophil elastase, IL-1 beta, and TNF-alpha concentrations were higher in those with detectable bacterial pathogens. Participants with absent inner dynein arm and microtubular disorganization had similar inflammatory profiles compared with participants with outer dynein arm defects. Conclusions: In this multicenter pediatric PCD cohort, elevated concentrations of sputum proteases and cytokines were associated with impaired lung function and structural damage as determined by chest computed tomography, suggesting that sputum inflammatory measurements could serve as biomarkers in PCD.
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