Understanding the Key Roles of pH Buffer in Accelerating Lignin Degradation by Lignin Peroxidase

pH buffer plays versatile roles in both biology and chemistry. In this study, we unravel the critical role of pH buffer in accelerating degradation of the lignin substrate in lignin peroxidase (LiP) using QM/MM MD simulations and the nonadiabatic electron transfer (ET) and proton-coupled electron transfer (PCET) theories. As a key enzyme involved in lignin degradation, LiP accomplishes the oxidation of lignin via two consecutive ET reactions and the subsequent C-C cleavage of the lignin cation radical. The first one involves ET from Trp171 to the active species of Compound I, while the second one involves ET from the lignin substrate to the Trp171 radical. Differing from the common view that pH = 3 may enhance the oxidizing power of Cpd I via protonation of the protein environment, our study shows that the intrinsic electric fields have minor effects on the first ET step. Instead, our study shows that the pH buffer of tartaric acid plays key roles during the second ET step. Our study shows that the pH buffer of tartaric acid can form a strong H-bond with Glu250, which can prevent the proton transfer from the Trp171-H center dot+ cation radical to Glu250, thereby stabilizing the Trp171H center dot+ cation radical for the lignin oxidation. In addition, the pH buffer of tartaric acid can enhance the oxidizing power of the Trp171H center dot+ cation radical via both the protonation of the proximal Asp264 and the second-sphere H-bond with Glu250. Such synergistic effects of pH buffer facilitate the thermodynamics of the second ET step and reduce the overall barrier of lignin degradation by similar to 4.3 kcal/mol, which corresponds to a rate acceleration of 103-fold that agrees with experiments. These findings not only expand our understanding on pH-dependent redox reactions in both biology and chemistry but also provide valuable insights into tryptophanmediated biological ET reactions.

Automated summary

pH buffer plays a critical role in accelerating the degradation of lignin substrate in lignin peroxidase (LiP). The pH buffer of tartaric acid stabilizes the Trp171H·+ cation radical and enhances the oxidizing power of the enzyme, facilitating the thermodynamics of the reaction and increasing the rate of lignin degradation. These findings provide valuable insights into pH-dependent redox reactions and tryptophan-mediated biological electron transfer reactions.