Matrix metalloproteinase 9 gene-MMP9-DNA methylation status in Turkish schizophrenia patients

Objectives: Schizophrenia is a neuropsychiatric disease caused by disorders in brain development due to genetic and environmental factors. The interactions and mechanisms of the two main etiological factors are not fully understood despite neurobiological, clinical and genetic research and more research is needed. In this study, we aimed to investigate the DNA methylation differences in the matrix metalloproteinase 9 gene (MMP9) between schizophrenia and healthy control groups.Methods: Our study investigates DNA methylation patterns in the MMP9 gene in peripheral blood cells of schizophrenia patients. For this purpose, pyrosequencing method was used to analyze DNA methylation status of C-phosphate-G (CpG) regions in the 7th exon of MMP9 in 40 schizophrenia patients and 32 healthy individuals who admitted to our university hospital's psychiatry clinic.Results: In our results, it has been shown that there is a difference between MMP9 gene DNA methylations between healthy individuals and schizophrenia patients. Significant differences were found in the MMP9 gene exon 7 region in CpG 7-3, CpG 7-4, CpG 7-5 positions and mean methylation patterns between these two groups. Conclusions: This study provided evidence that DNA methylation differences may exist between schizophrenia patients and healthy individuals, and emphasized the epigenetic aspect of the disorder.

Automated summary

This study investigated the DNA methylation differences in the MMP9 gene between schizophrenia patients and healthy individuals. The results showed significant differences in CpG 7-3, CpG 7-4, and CpG 7-5 positions as well as the overall methylation patterns in the 7th exon of MMP9 between these two groups. The study provided evidence for the potential DNA methylation differences in schizophrenia and emphasized the importance of the epigenetic aspect of the disorder.